Effect of Nano Silver Particles (AgNps) on ESBL Producing Klebsiella spp. and E. coli in Pets

Abstract

In the present study, the prevalence of ESBL producing E. coli and Klebsiella spp. was investigated in different samples obtained from pet animals. Samples included fecal swabs (n=61), wound swabs (n=17), and urine samples (n=42). Samples were collected from Al-Shaab Veterinary Hospital, Surgery and Medicine Departments at Faculty of Veterinary medicine, Cairo University as well as animal laboratories in Cairo and Giza from March to December 2019 MacConkey agar supplemented by Ampicillin was used for the isolation of ESBL-producing isolates which facilitate screening of the isolates. The antibiogram was assessed for E. coli and Klebsiella spp. isolates. Ampicillin showed the highest percentage for resistance in both E. coli and Klebsiella spp. The most prominent groups in resistance pattern were cephalosporins first and third generation represented by cephalexin (92%, 76%), cefotaxime (87%, 48%), ceftazidime (81%, 60%), ceftriaxone (71%, 40%) for E. coli and Klebsiella Spp., respectively. Moreover, amoxicillin clavulanic acid indicated 63% resistance for E. coli isolates and 60% for Klebsiella spp. The overall result of the antibiogram revealed that about 29 isolates (E. coli =20, Klebsiella spp.=9) were suspected to be ESBL producing isolates. ESBL production was identified using Double Disk Synergy Test (DDST). Three antibiotics were used for DDST ceftriaxone (30µg), Amoxicillin-clavulanic acid (20/10µg), and ceftazidime (30µg). Discs were placed at a distance of 1.5cm. ESBL positive organism are showing development of the inhibition zone towards the clavulanate disc at 37°C after 24-hours-incubation. In our study, detection of suspected ESBL-producing isolates revealed that 23 isolates (E. coli=17, Klebsiella=6) out of the 120 samples (23/120) of the current study representing 19.6 % of the total sampleswere ESBL producing isolates.