The Potential Role of Human Derived Amniotic Membrane Graft in the Repair of Induced Tendon Tear in Adult Male Albino Rat: Histological Study

Abstract

endon injuries are considered one of the most common injuries of human body. They may be either complete tendon ruptures or incomplete injuries such as tendinitis. These injuries cause large health care costs and individual disability. Although there are current medical treatments for most of these conditions, tendon injuries treatment is still among the challenges in medicine. Continuous efforts are still needed to enhance the effectiveness of the treatments and to accelerate recovery to the normal biochemical properties of the tendon. The human amniotic membrane is the inner-most layer of the placenta. It is non-vascular, non-immunogenic tissue that contains extracellular collagen matrix. It has been recently used in wound healing, and tissue re‐modulation or regeneration. It was reported to promote cellular migration and trophic factors secretion at the site of injury which consequently might promote healing. The current experiment was carried out to study the effect of human amniotic membrane graft application on the repair of induced Achilles tendon tear in adult male albino rats. Three human placentas were harvested from the obstetrics and gynecology hospital for preparation of the human amniotic membrane grafts. The grafts were examined by the light microscope after being stained by Hematoxylin and Eosin, Masson's trichrome, VEGF immunohistochemical staining and toluidine blue-staining for semithin sections. They were also examined by the transmission electron microscope.