Role of Long Non Coding RNA in Prognosis of Acute Myeloid Leukemia

Abstract

was detected between Lnc-HOTAIR and miR-193a, which found that the expression level of the miR-193a in AML patients showed to be a tumor suppressor gene by 0.375 folds. Moreover, the expression level of c-kit gene was up-regulated by 3 folds in AML patients compared to healthy control. These data reveal a critical role for HOTAIR, miR-193a and c-kit axis as poor outcome in Acute Myeloid leukemogenesis.

Acute myeloid leukemia (AML) is a heterogeneous hematopoietic malignancy, which presents unrestricted self-renewal and proliferation. Molecular biomarkers open the door to improve early disease diagnosis. Hox transcript antisense intergenic RNA (HOTAIR) can be considered as a potential cancer biomarker in AML. It is a long non-coding RNA, which is abundantly expressed and plays oncogenic roles in various human solid tumors and hemopoietic malignancies. Epigenetic of tumor-suppressive microRNAs (miRNAs) is also a key of oncogenic mechanism for the activation of oncogenes in tumors. Aberrant activation of c-kit proto-oncogene contributes to abnormal cell proliferation by altering the tyrosine kinase signaling and constitutes a crucial impetus for leukemogenesis. Using real-time quantitative reverse transcription-PCR (qRT-PCR) to evaluate Lnc-HOTAIR expression level in de novo AML patients compared with healthy controls and realize the expression level of both miR193a and c-kit gene to find out the association between the expression of these two targeting genes on Lnc-HOTAIR expression level. The obtained data suggested that the expression level of Lnc-HOTAIR was significantly upregulated by four folds in peripheral blood samples of de- novo AML patients compared to normal controls. Therefore, it may represent a poor prognostic tool for AML. In addition, a non-significant negative correlation