Molecular and Biological Studies of the Effect of Simvastatin and Green Tea Extract and\ or Gamma Radiation on Cancer Cells

Abstract

The current study was investigated the anticancer effect of Epigallocatechin-3-gallate (EGCG), Simvastatin, and Gamma radiation individually on myeloma cell line and Ehrlich carcinoma cells (in vitro), in addition to their effect on the solid tumor of the right thigh of mice (in vivo). In the preliminary study on myeloma cells, seven groups were conducted to test and select the dose of EGCG which kill around 50% of cells and then designed nine groups to study the EGCG and its effect in combination with simvastatin (Dual therapy) and\or gamma radiation or both (Triple therapy). Regarding preliminary study on Ehrlich carcinoma cells, twenty groups were conducted to study the different doses of each of EGCG (five doses), Simvastatin (five doses) and Gamma radiation (six doses) and select the dose for each treatment to continue our study. For more confirmation about the potency of those regimens, the solid tumor was induced by intramuscular injection of EC cells in the right thigh of the targeted mice. Magnetic resonance imaging device 1.5 Tesla used for radiology imaging of the normal and ascites-bearing mice under anesthesia. The animals with solid tumors were followed up daily for recording deaths, tumor size, rate of inflammation, and gangrene in the tumor area of the hind limb. Magnetic resonance imaging showed the intraperitoneal ascites and the soft organs (liver, kidney, and intestine), which were bright in the case of the ascites-bearing mouse than the normal control. The doses of Simvastatin, EGCG and Gamma radiation induced cytotoxicity about 50% of cells on myeloma cells and Ehrlich carcinoma cells as the