MICROBIAL DEGRADATION OF TEXTILE DYES IN WASTEWATER

Abstract

In this study; a survey was carried out on Ten wastewater samples used as a consortium for removing about five dyes with adjusting bio decolorization factors and twelve bacterial isolates were isolated from the best consortium and selected the ideal 3 isolates in decoloring dyes in high percentage and then were identified by Morphological , physiological characteristics as well as 16SrRNA. LC/MS was used to detect the biodegradation by-products of Direct red81 dye by the most active bacterial isolates during the study. And because there is a great interest in decolorization of the textile dyes and there are a large number of microorganisms doing this process, the yeast was used to break down the dyes as it accumulates the dyes within their succulent gaps and does not break them down so, in this study, we studied the effect of yeast on the methylene blue dye and thus this has been compared Between yeast and bacteria in getting rid of textile dyes. Results of the present study are summarized in the following points: 1- The Physico-chemical characteristics of different wastewater samples were evaluated. PH, TDS, EC, Carbonate, HCO3-, Major cations, Major anions, Trace metals, BOD, COD, DO, Phenol, and total organic carbons for all the collected samples. the values were unacceptable. 2- Ten wastewater samples were preliminarily screened for their maximum decolorization of Methylene blue dye and S10 show the highest Decolorization % 87, 93, and 97 % after 1hr, 2hrs, and 24hrs respectively. 3- Screening of different five media for growing bacteria used for decolorization. Data showed that the enrichment medium (nutrient broth) medium no.1 is the optimum media used between five types of media followed by medium no.3, media no.2, medium no.4, and medium no.5.