Analytical Investigation on Certain Antihypertensive Drugs

Abstract

The proposed thesis composed of seven parts involving introduction, literature review, proposed analytical work and references with Arabic summary. Part I: General Introduction It includes information about hypertension, types of hypertension, risk factors, management of hypertension and general information about antihypertensive drugs. Part II: Literature Review It involves different reported methods for determination of Telmisartan, Xipamide and Triamterene in pure and dosage form. Part III: Chromatographic methods Section A: Stability indicating UPLC method for determination of Telmisartan in presence of its oxidatitive degradate in bulk drug and pharamcutical formulation. Stability indicating UPLC method for determination of Telmisartan in-presence of its oxidative degradate in pure form and pharmaceutical dosage form was performed under certain conditions: Agilent Zorbax Eclipse Plus C8 (50 mm × 2.1 mm, 1.8 μm) as stationary phase. Mobile phase: acetonitrile: 20 mm phosphate buffer ratio (75: 25 V/V) (0.2 M Heptane sulphuric acid was added) to adjust pH (3), detection wavelength: 231.4 nm, flow rate: 0.2 ml min-1 and injection volume: 1.0 µL with structure elucidation of the proposed oxidative degradation product using IR, NMR and LC/MS. Section B: Determination of Xipamide and Triamterene in pure and dosage form by UPLC method in bulk drug and pharamcutical formulation. Determination of Xipamide and Triamterene by UPLC using Agilent Zorbax Eclipse Plus C8 (50 mm × 2.1 mm, 1.8 μm) as stationary phase, (acetonitrile: water) ratio (70: 30 V/V) pH is adjusted to 3 by 0.2 M acetic acid as the mobile phase, flow rate: 0.2 ml min-1, Injection volume: 1.0 µL and Detection wavelength: 231.4 nm. Section C: Stability indicating TLC densitometric method for determination of Telmisartan in presence of its oxidative degradate in bulk drug and pharmaceutical formulation. Stability indicating TLC densitometric method for determination of Telmisartan in-presence of its oxidative degradate was achieved by using chloroform: methanol: ammonia (8:2:0.2 V/V) as the mobile phase and silica gel plates as stationary phase at 254.0 nm. Linearity range for Telmisartan was (2.0 - 12.0 µg band-1) with Rf = 0.50. Section D: TLC densitometric method for the determination of Xipamide and Triamterene in bulk drug and pharmaceutical formulation.