SOME STUDIES ON ADEN0-3 VIRUS vACCINE

Abstract

The role of Adeno-virus as an etiologic agent of bovine respiratory and enteric diseases has much attention in recent years, and the Adeno-virus are responsible for producing great losses to the livestock industry in many ways.

I I I In the present study aiming to prepare and evaluate inactivated I I I monovalent freeze dried Adeno-virus vaccine for control the spread of this I I viral agent in Egypt, and to assist in saving animal industry in Egypt.

The susceptibility of different cell cultures (BK, MDBK, VERO, CER) for cultivation of Adeno-virus was studied to choose the best cells for vaccine production. :MDBK was the culture of choice as it produces high titre and was in preparation.

Binary ethyleneimine was used for the inactivation of Adeno-virus, different concentration of Binary ethyleneimine at different times were made r to detect the suitable concentration of BEI at a proper time for complete

inactivation of Adeno-virus .