Effect of Blood Contamination on bioactivity of Nanoparticles hydroxyapatite with different concentration as Root End Filling Material (In Vitro Study)

Abstract

Endodontic microsurgery is commonly used for treatment of teeth with persistent pathosis or where conventional retreatment methods are contraindicated. After root resection and retrograde cavity preparation, retro-filling material is used to fill the cavity. MTA is the gold standard material for this purpose so in our study, we compared the IRM with different concentration of HAn with it. During the application of retro-filling material, blood contamination could happen which might affect the biological properties of the used material. The aim of this study was to evaluate the effect of blood contamination taking place during setting of IRM with different concentration of HAn comparing with MTA as retro-filling material on the bioactive properties. Forty-eight extracted single rooted sound human anterior teeth were prepared and obturated. Roots were resected, retrograde cavities were ultrasonically created and the materials under research were inserted. Teeth were divided into 2 groups (n=24) according to the liquid in which setting of the materials took place. Group I was allowed to set in blood. Group II was allowed to set in