The potential link between obesity, synbiotics intake and inflammasomes in an animal model

Abstract

Obesity is considered a major health problem and a leading cause of many chronic diseases. The dysbiosis of intestinal microbiota occurred with obesity contributes with the chronic inflammatory status of obesity. In this context, our study together with the recent concern in the nutrition field research demonstrated the effect of symbiotic administration on improving the inflammatory status associating obesity to elicit its potential beneficial effect as a treatment or preventive agent in obesity and obesity related disorders. The aim of our work was to study the potential involvement of inflammasomes as an indicator for the efficacy of synbiotics in alleviating diet-induced obesity. This study was conducted at Medical Biochemistry department, faculty of Medicine from January 2019 to October 2020. Housing was at the animal house at MASRI research center, Faculty of Medicine, Ain Shams University (Abbassia, Cairo, Egypt) where twenty -four adult male Wistar rats randomly divided into four groups (6 rats /group): normal control group (receiving normal diet), HFD (high fat diet) group, normal group receiving synbiotics, and HFD rats receiving synbiotics, The animals were maintained at a temperature (22 ± 2°C), relative humidity (55 ± 5%), 12 h light/dark cycle at 5:00 am–5:00 pm and good ventilation with free access to food and water. The cages were cleaned daily.