EFFECT OF ANAESTHESIA ON THE CYTOKINE RESPONSES TO ABDOMINAL SURGERY

Abstract

All the patients were premedicated with diazepam 10 mg orally and atropine sulfate 1 mg intramuscularly one hour before the begining of induction with anaesthesia. All the patients were given between 500 to 700 ntl3 saline 0.9% as an infusion before the start of operation. Monitoring in the form of continuous ECG, systolic and diastolic arterial blood presure, end tital expiratory Co and transcutaneous arterial O saturation were used for all cases. Blood samples were taken via a wide bore cannula (18 G) in a central vein as follow : (1) A base-line sample: At the night before the day of operation. (2) After induction of anaesthesia and _before skin incision. (3) At the end of operation. (4) 6 hours after the end of operation. We measured the level of:• (1) Interleukin-19 (IL-19). (2) Interleukin-2 (IL-2) (3) Interleukin-6 (IL-6). (4) Interleukin-8 (IL-8) (5) Tumour Necrosis Factor-a (TNF-a). by using the commercially available enzyme amplified sensitivity immunoassays (EASIA, Medginix Diagnostics S.A., Fleurus, Belgium). This was to compare the level of these factors in the same group by using the paired-t-test and also to compare between the 33 groups by using the ANOVA test.