STUDY OF DIFFERENT TOXICOLOGICAL METHODS FOR ANALYSIS AND DETECTION OF SILDENAFIL

Abstract

Sildenafil is considered to be a revolutionary drug for erectile dysfunction (ED). The drug is involved directly or indirectly in some deaths. The present study was designed to 1) reveal which of the different toxicological analytical methods is more suitable for the identification of sildenafil, 2) estimate the postmortem drug concentration in some tissues of rats injected intraperitoneally with two different doses of the drug. The results obtained can be summarized as follow: 1) Color tests revealed that some chemical reagents are suitable for the identification of sildenafil (e.g. Marquis reagent, Frohde's reagent, Forrest reagent, cobalt thiocyanate and cobalt nitrate / ammonia). 2) Different coloring reagents were sprayed on the chromatoplates of TLC and the results revealed that iodoplatinate reagent and palladium chloride reagent may be considered specific for the identification of sildenafil.