Anti-Angiogenic Efficacy of Chitosan Nanoparticles as Drug Delivary on Mice Bearing Ehrlich Carcinoma and Subjected to γ-Irradiation

Abstract

The present study, was carried out to evaluate the role of chitosan nanoparticles as a drug carrier for 5-fluorouracil and to evaluate the anticancer activity of 5FUCNPs either alone or in combined with γ-radiation . As tumor have systemic effects on the host , the antioxidant status for tumor , liver, kidney and spleen have been studied .More and above , histopathological and apoptotic examination of tumor , liver , kidney and spleen tissues were performed to evaluate the histo-pathological and apoptotic changes. In vitro the cytotoxicity of nanoparticles not only led to the death of Ehrlich carcinoma cells, but also led to the burst of these dead cells at certain doses. Nanoparticles led to rupture of cells content after their death .The median lethal concentration of 5FUCNPs was 20μg/ml for Ehrlich carcinoma cells. In vivo experiments were performed on healthy Ehrlich-bearing female Swiss albino mice. Mice were randomly assigned and equally divided into 5 experimental