FEATURES, OF HEALING EVENTS IN PERIODONTAL DEFECTS AFTER GUIDED TISSUE REGENERATION TREATMEN

Abstract

Periodontitis is an inflammatory disease characterized by destruction of the supporting periodontal tissue. Standard treatment techniques do not result in significant periodontal regeneration for the lost periodontal tissue. Devices and bioactive substances have been developed to favor repopulation of the periodontal defects by periodontal progenitor cells. Numerous clinical and experimental studies have demonstrated that regeneration is biologically possible and clinically feasible. The objective of the present investigation is to study the nature of regenerating tissues after the use of guided tissue regeneration using non-absorbable membrane. Twenty patients suffering from adult periodontitis were selected free from any systemic diseases and they had bilateral intrabony or furcation defects in posterior region. The lesions were treated through GTR by using ePTFE membranes. The membrane was retrieved in one side after two weeks and in the other side after four weeks. Specimens from the membrane (3mm) were prepared and examined by scanning electron microscope (SEM) at the inner and outer surfaces. I' Tissue specimens from the regenerating tissue inside the defect (1mm) / was dissected away to be examined by transmission electron microscope (TEM). Scanning electron microscopic evaluation of the inner and outer surface of the membrane retrieved after two weeks revealed variable amount of organic deposits precipitated over the ePTFE membrane. These organic deposits consisted, numerous erythrocytes, inflammatory cells, bacterial cells and fibroblast like cells but epithelial cells were not adhering to the membrane