Detection of antibiotic and disinfectant resistance genes in E.coli isolated from chicken

Abstract

E.coli constitute a threat to poultry industry, poultry producers and related industries, that make sever economic loss in a year, as well as the most frequent foodborne gastrointestinal disease transmitted to humans.

This work was carried out to detect the antibiotics and disinfectants resistance genes among E coli isolated from chickens. The incidence of E.coli in chicken was 34%. E.coli isolated were serologically identified commonly serogroups were O128, O111, O44, O158 and O2.

E.coli revealed that 100% of isolates were resistant to ampicillin, erythromycin and sulfamethoxazole - trimethoprim, while were sensitive for colistin sulphate and levofloxacin with 73.53% and 38.23%respectively.

Using PCR technique antibiotic resistance genes as tetA gene was tested for isolated E.coli and detected by incidence rate 91.18%. qac resistance genes resembling as qacED1 and qacA/B genes were detected in isolated E.coli 70.6% and 14.7% respectively , while qacC/D gene wasn’t detected in all E.coli isolates