Screening and Confirrmatory methods for detection of Brucellosis in milk of some dairy animals in Behera province

Abstract

The difficulty of collecting blood for detection of Brucellosis needed the using of milk and milk serum as easier method on this respect. In an aim to determine the most suitable test for the diagnosis of . Brucellosis in milk, a total of 54 milk samples were collected from Friesian dairy cows belonging to a herd with a history ofbrucellosis. .J These were subjected to the MRT and ELISA milk test. Whey was separated from milk samples for carrying out WBAPAT, WRBPT, WRiv.T and WELISA test The ELISA milk test was taken as a reference standard for evaluating other tests. The sensitivity obtained were (48.148%), (65.52%), (68.97%), (89.66%) and (92.308%) for MRT, WRBPT,WBAPAT, WRiv.T and WELISA test, respectively. The specificity obtained were (72.222%), (72.22%), (100.00%), (61.11%) and (93.75%) for MRT, WRBPT, WBAPAT, WRiv.T and WELISA test, respectively. The agreement calc'ulated were (48.1%), (59.30%), (70.40%), (68.50%) and (79.6%) for MRT, WRBPT, WBAPAT, WRiv.T and WELISA test,

respectively. The results of different tests were discussed. It was concluded that the milk ELISA was relatively more sensitive and specific and the WELISA test was a good substitute to the ELISA milk test. Serum tests as BAPA, RBPT and Riv. T need further investigations to be adapted for whey.