Production and Evaluation of Local Radioimmunoassay System for Estimation of Ferritin in Human Serum

Abstract

The radioimmunoassay (RIA) is the most widely used sensitive micro analytical technique for evaluating a variety of biological and medicinal substances. The applicability, ease of use, and cost-effectiveness of RIA have paved the path for the last four decades of medical research advancement. Ferritin protects lipids, DNA, and proteins from the potentially harmful effects of iron while also making iron available for critical cellular functions. Iron homeostasis is dependent on ferritin, which is the primary iron storage mechanism Low blood ferritin levels suggest diminished iron storage and is one of the most often requested examinations in both primary and secondary care. Serum ferritin levels can be elevated for a variety of reasons, including iron overload, inflammation, liver or renal illness, Covid-19 infection and cancer. Aim of the work Establish and develop a radioimmunoassay system for determination of ferritin level in human serum. Materials and methods 1- Extraction and purification of ferritin Extraction and purification of ferritin from human spleen which can be used as the corner stone to establish the RIA system.