Glycosphingolipids and its related enzyme In schistosomiasis

Abstract

Schistosoma is one of the common diseases in Egypt and many previous studies have been published about the induction of Schistosoma in experimental animals. Mice have the highest incidence of Schistosoma infection in the previous studies. The purpose of the present work is to investigate the effect of Schistosoma infection on gangliosides, and its related enzyme, arylsulfatase A (ASA). This study was also applied: on arlysulfatase B (ASB) as being one type of arylsulfatases catalyzing the hydrolysis of the same substrate, p-nitrocatechol sulfate (PNCS). Animals were divided into six groups according to the period of infection (15-90 days) and each group had its control one at each time interval. The animals from both control and infected groups were sacrified and liver was quickly removed and immediately homogenized to isolate gangliosides, ASA, and ASB. Gangliosides were isolated by mixture of methanol and chloroform, partially purified by gel filteration and then were identified by TLC. Arylsulfatases A and B were separated and fractionated from liver homogenates on ion exchange chromatography by DE-52 column for determination of their catalytic activity and the study of kinetic properties. The following results have been achieved