New chitosan derivatives inspired on heterocyclic anhydride of potential bioactive for medical applications

Abstract

In the present work new chitosan derivatives inspired heterocyclic anhydride were prepared to improve the bi ological activities of chitosan via imidization reaction of chitosan (CS) and N-(1,3-dioxoisoindolin-2-yl)-1,3- dioxo-1,3-dihydroiso-benzofuran-5-carboxamide (5) to yield amic acid CS-6 at room temperature and imide CS-8 thermally. However, the reaction between (CS) and anhydride (5) in presence of sodium tripolyphosphate (TPP) or Poly (ethylene glycol) diglycidyl ether (PEGDG) at room temperature yielded CS-6 NPs and CS-7 respec tively. The structure of new chitosan derivatives was characterized using morphological and spectroscopic anal yses. From evaluation of the biological activities, the greatest enzymatic inhibitory for trypsin and α chymotrypsin revealed by CS-7 at 88.33 ± 2.27 and 79.63 ± 3.16% respectively. Furthermore, the highest inhibi tion zones, (MIC) and (MBC) against S. aureus and B. subtilis recorded by CS-6 NPs at 21 ± 0.75, 22 ± 0.98 mm, 19.5, 19.5, 38 and 38 ppm respectively. Additionally, CS-8 displayed the best cell growth inhibition against vero cell line at 93.17 ± 0.29%.