Radioimmunoassay of 5 alpha-pregnane-3,20-dione. A metabolite of placental progesterone
Zwirner, M; Elhao, Mounir M Fawzy; Bopp, F C; Klemm-Wolfgram, E; Handschuh, D; Voelter, W; Schindler, A E;
Abstract
A radioimmunoassay (RIA) procedure has been developed for the measurement of 5 alpha-pregnane-3,20-dione (DHP) in human plasma after ether extraction of the plasma-samples, followed by column chromatography. The antiserum was generated in rabbits with 6 alpha-carboxyethylmer-captoprogesterone-BSA conjugate. The high affinity (Ka = 3.72 X 10(9) l/mol) antiserum binds 40% of 40 picograms of tritiated 5 alpha-pregnane-3,20-dione at working dilutions of 1 : 2800. Negligible cross-reactivity of the antiserum was detected with 5 beta-pregnane-3,20-dione (1.8%). Other hydroxylated pregnanes showed minor cross-reactivity (5-40%). The cross-reacting steroids were all separated from pregnanedione by one chromatographic step. The plasma levels of 5 alpha-pregnane-3,20-dione were measured from 22nd to 42nd week of normal pregnancy and compared to published data.
Other data
| Title | Radioimmunoassay of 5 alpha-pregnane-3,20-dione. A metabolite of placental progesterone | Authors | Zwirner, M; Elhao, Mounir M Fawzy ; Bopp, F C; Klemm-Wolfgram, E; Handschuh, D; Voelter, W; Schindler, A E | Keywords | 5α-Pregnane-3,20-dione;Pregnancy;Radioimmunoassay | Issue Date | 1983 | Journal | Archives of gynecology | Volume | 233 | Start page | 229 | End page | 240 | ISSN | 0170-9925 | DOI | 10.1007/BF02133796 | PubMed ID | 6660916 | Scopus ID | 2-s2.0-0020572430 |
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