Morphological and Molecular characterization of Physical and Chemical Mutations on Durado Plum Cultivar
Heba. M. Khater; Y.S. Abd Elaziz; A. F. Khafaga; A. B. Abdel-Razik; S. A. Ibrahim; Khaled Abdelfattah Metwally;
Abstract
Improving the marketability of plum cultivars; requires the production of large and high
fruits. Breeding by amutation on plums can be used to generate genetic diversity that allows
the breeder to screen the mutants for superior quality and quantity fruits. The study was
conducted over two-years (2021-2022) in a private farm. It aimed to investigate the impact of
various doses of gamma irradiation acting as physical mutagens (20, 30, 40, and 50 Gy) of
gamma irradiation from a cobalt (60CO) source, as well asthe effects of (ethyl methane
sulphonate EMS) as chemical mutagens with different concentrations at (0.05, 0.1 and
0.2%)on inducing mutations in the Durado plum cultivar. The study on survival percentage
after grafting, morphology and molecular characteristics, identifies new mutated genotypes at
the molecular level and finally evaluate some of the new genotyping. Utilizing the ISSR
technique of compare and relationships between control with Mutagenic plants based on a
dendrogram. The results of control gave the highest value on survival percentage after grafting.
40 Gy and EMS 0.2% given the best results in morphology characteristics of the tree in
compare with the control. Additionally, the study of relationships and correlation coefficient
based on a dendrogram revealed that the relationships between 20 Gy and EMS 0.05% and the
control are similar. While 40 Gy and EMS 0.2% is very far in relationship with control.
fruits. Breeding by amutation on plums can be used to generate genetic diversity that allows
the breeder to screen the mutants for superior quality and quantity fruits. The study was
conducted over two-years (2021-2022) in a private farm. It aimed to investigate the impact of
various doses of gamma irradiation acting as physical mutagens (20, 30, 40, and 50 Gy) of
gamma irradiation from a cobalt (60CO) source, as well asthe effects of (ethyl methane
sulphonate EMS) as chemical mutagens with different concentrations at (0.05, 0.1 and
0.2%)on inducing mutations in the Durado plum cultivar. The study on survival percentage
after grafting, morphology and molecular characteristics, identifies new mutated genotypes at
the molecular level and finally evaluate some of the new genotyping. Utilizing the ISSR
technique of compare and relationships between control with Mutagenic plants based on a
dendrogram. The results of control gave the highest value on survival percentage after grafting.
40 Gy and EMS 0.2% given the best results in morphology characteristics of the tree in
compare with the control. Additionally, the study of relationships and correlation coefficient
based on a dendrogram revealed that the relationships between 20 Gy and EMS 0.05% and the
control are similar. While 40 Gy and EMS 0.2% is very far in relationship with control.
Other data
| Title | Morphological and Molecular characterization of Physical and Chemical Mutations on Durado Plum Cultivar | Authors | Heba. M. Khater; Y.S. Abd Elaziz; A. F. Khafaga; A. B. Abdel-Razik; S. A. Ibrahim; Khaled Abdelfattah Metwally | Keywords | Prunussalicina;physical mutagen;chemical mutagen;molecular genetic | Issue Date | Jun-2023 | Publisher | Horticulture Research Institute, ARC | Journal | Horticulture Research Journal | Volume | 1 | Issue | 1 | Start page | 148 | End page | 160 | DOI | 10.21608/hrj.2023.307659 |
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| Morphological and Molecular characterization of Physical and chemical mutations on durado plum cultivar.pdf | 1.35 MB | Adobe PDF | Request a copy |
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