1-14C linoleoyl-COA desaturation into diverse lipid classes of dracocephalum moldavica cotyledons
Abdel-Reheem or Abdelreheem, Mohammed; Hildebrand, David;
Abstract
To elucidate factors that contribute to the high 18:3 content of Dracocephalum moldavica (D. moldavica), a pulse-chase study of radiolabled 14C linoleoyl-CoA was carried out at an early and late stage of embryo triglyceride (TG) synthesis. In D. moldavica 14C-18:2 incorporation in PC increased and 14C-18:3 decreased, meanwhile the opposite was observed in TG (the incorporation of 14C-18:3 in TG is two-fold that of 14C-18:2 in PC). This suggests that both ω-3 desaturase is effectively desaturating 14C -18:2 in PC and acyltransferases are selectively transferring 14C -18:3 into TG. Also 14C -18:3 increased in D. moldavica TG indicates that most of the 18:2 was desaturated to 18:3 before transfer to the free fatty acid/acyl-CoA pool in D. moldavica.
Other data
| Title | 1-14C linoleoyl-COA desaturation into diverse lipid classes of dracocephalum moldavica cotyledons | Authors | Abdel-Reheem or Abdelreheem, Mohammed ; Hildebrand, David | Keywords | Dragonhead | Fatty acids | Lipid classes | Issue Date | 25-Oct-2013 | Journal | Life Science Journal | ISSN | 10978135 | Scopus ID | 2-s2.0-84886900166 |
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