The role of E. coli infection on IBD antibodies in chicken detected by ELISA titers
Gamal Rayan; H.H. Rezk;
Abstract
Background: Several factors causing immune suppression in chickens would lead to immune deficiency, Escherichia coli (E. coli) infection is one of these important determinants, for example, infectious bursal disease (IBD), infectious bronchitis indirect, Newcastle disease, H5 and H9 an acute illness that is extremely communicable and infectious and affects young chicks. The current study aims to determine the level of antibodies in chicks infected with E. coli.
Methods: The chicks were divided into two groups: the first group vaccinated chicks against IBD disease at the age of one day and ten days, compared to the second group vaccinated chicks at the age of 10 days only. Five thousand one-day-old Fayoumi chicks were raised in the experiment and divided into two groups for comparison against IBD virus antibodies.
Result: The study revealed that infectious bursal disease (IBD) infected chickens and inoculated with E. coli pre or concurrent to infection had lower antibody titers in both groups whether vaccinated at one day old or not, the level of antibodies to IBD disease is low in chicks, whether vaccinated or not vaccinated at one day of age, but when chicks were vaccinated on the ten day old of age with a weak vaccine IBD disease for both groups, it was found that the chicks that were vaccinated on the first day had a higher level of antibodies to IBD disease than the unvaccinated chicks at the age of one day. Fayoumi chicks, mean ELISA titers at one day was (1556±132) increased sharply to (3440±960) at 15 days of age and reach (5596±830) at 20 days of age for group 1 compared to group 2 was the results, mean ELISA titers at one day was (1470±187) increased slightly sharply to (2710±779) at 15 days of age and reach (3560±670) at 20 days of age. All samples tested positive for E. coli at one day of age, but turned negative at 15 and 20 days of age. Additionally, the indirect test for Infectious Bronchitis showed a result of 11459 ± 250 at one day of age. In addition, showed that the effect of post inoculation of E. coli on the level of ND antibodies was studied in group 1 with E. coli infection revealed that the HI titer of all subgroup had arranged of geometric means varied was (7.8), in group 2 was ranged (7) where the effect was not significant.
Methods: The chicks were divided into two groups: the first group vaccinated chicks against IBD disease at the age of one day and ten days, compared to the second group vaccinated chicks at the age of 10 days only. Five thousand one-day-old Fayoumi chicks were raised in the experiment and divided into two groups for comparison against IBD virus antibodies.
Result: The study revealed that infectious bursal disease (IBD) infected chickens and inoculated with E. coli pre or concurrent to infection had lower antibody titers in both groups whether vaccinated at one day old or not, the level of antibodies to IBD disease is low in chicks, whether vaccinated or not vaccinated at one day of age, but when chicks were vaccinated on the ten day old of age with a weak vaccine IBD disease for both groups, it was found that the chicks that were vaccinated on the first day had a higher level of antibodies to IBD disease than the unvaccinated chicks at the age of one day. Fayoumi chicks, mean ELISA titers at one day was (1556±132) increased sharply to (3440±960) at 15 days of age and reach (5596±830) at 20 days of age for group 1 compared to group 2 was the results, mean ELISA titers at one day was (1470±187) increased slightly sharply to (2710±779) at 15 days of age and reach (3560±670) at 20 days of age. All samples tested positive for E. coli at one day of age, but turned negative at 15 and 20 days of age. Additionally, the indirect test for Infectious Bronchitis showed a result of 11459 ± 250 at one day of age. In addition, showed that the effect of post inoculation of E. coli on the level of ND antibodies was studied in group 1 with E. coli infection revealed that the HI titer of all subgroup had arranged of geometric means varied was (7.8), in group 2 was ranged (7) where the effect was not significant.
Other data
| Title | The role of E. coli infection on IBD antibodies in chicken detected by ELISA titers | Authors | Gamal Rayan ; H.H. Rezk | Issue Date | 1-Nov-2025 | Journal | Indian Journal of Animal Research | Volume | 59 | Issue | 11 | Start page | 1923 | End page | 1927 | DOI | https://doi.org/10.18805/IJAR.BF-2000 |
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