Isolation and characterization of basil mosaic virus in Egypt
Marie, Eman; M. Magdy;
Abstract
Different viral infection symptoms were observed on leaves
of basil plants (Ocimum basilicum L.), growing at El-Monofia
Governorate, Egypt, during 2015–2016. The majority of
these symptoms were mosaic and leaf malformation. The
pathogenic agent of this disease is known as basil mosaic
virus (BsMV). The sensitive hosts to BsMV are Chenopodium
amaranticolor and Phaseolus vulgaris cv. (brown beans). The
biological properties of BsMV were found to be 10−4, 6 days
and 66 °C for dilution end point, longevity in vitro and thermal
inactivation point, respectively. The electron micrograph
of the isolated virus indicated this virus was found to be
isometric particles of approximately, 50 nm in diameter and
could be classified as tentative member of the Fabavirus. Realtime PCR detection by the Fab primer pairs was confirmed at
Ct value = 25.44 for the total cDNA isolated from infected Basil
plants and 27.94 for the cDNA of purified virus particles, while
CMV primer pairs showed no amplification for both samples.
According to the available reports this seems to be the first
record for basil mosaic virus in Egypt.
of basil plants (Ocimum basilicum L.), growing at El-Monofia
Governorate, Egypt, during 2015–2016. The majority of
these symptoms were mosaic and leaf malformation. The
pathogenic agent of this disease is known as basil mosaic
virus (BsMV). The sensitive hosts to BsMV are Chenopodium
amaranticolor and Phaseolus vulgaris cv. (brown beans). The
biological properties of BsMV were found to be 10−4, 6 days
and 66 °C for dilution end point, longevity in vitro and thermal
inactivation point, respectively. The electron micrograph
of the isolated virus indicated this virus was found to be
isometric particles of approximately, 50 nm in diameter and
could be classified as tentative member of the Fabavirus. Realtime PCR detection by the Fab primer pairs was confirmed at
Ct value = 25.44 for the total cDNA isolated from infected Basil
plants and 27.94 for the cDNA of purified virus particles, while
CMV primer pairs showed no amplification for both samples.
According to the available reports this seems to be the first
record for basil mosaic virus in Egypt.
Other data
| Title | Isolation and characterization of basil mosaic virus in Egypt | Authors | Marie, Eman ; M. Magdy | Keywords | basil mosaic virus; transmission electron microscope; fabavirus; realtime PC | Issue Date | 2017 | Journal | Archives of Phytopathology and Plant Protection | Volume | 50 | Issue | 13–14 | Start page | 687 | End page | 699 | ISSN | 03235408 | DOI | org/10.1080/03235408.2017.1368908 | Scopus ID | 2-s2.0-85028526020 |
Attached Files
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| Isolation and characterization of basil mosaic virus in Egypt.pdf | 1.95 MB | Adobe PDF | Request a copy |
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