Phenotypic diversity and molecular identification of the most prevalent anastomosis group of Rhizoctonia solani isolated from diseased faba bean plants
Mohamed Maha Helmy; Gado Emad; Helmy, Maha; El-Deeb Samir;
Abstract
One hundred and thirty one isolates of Rhizoctonia spp. were isolated from faba bean plants showing root rot and
stem canker collected from different fields in Delta region of Egypt. Forty six isolates were found to be polynucleate and the
remaining isolates were binucleates. According to morphological features of isolates, they were classified into 12 groups.
Polynucleate isolates were identified as Rhizoctonia solani (Kühn). The most aggressive isolate of R. solani was identified
according to sequences of ITS1-5.8S rDNA-ITS4 and the sequence was compared with Thanatephorus cucumeris
(teleomorphic phase) and other R. solani (NCBI GenBank). Sequence and comparison revealed that this isolate is R. solani
AG4-HGI. Anastomosis test carried out between molecular identified isolate and 11 randomly chosen isolates resembles all
groups of polynucleate R. solani. All tested isolates were completely fused between each other indicating that the prevalent AG
of R. solani on faba bean is AG4-HG1.
stem canker collected from different fields in Delta region of Egypt. Forty six isolates were found to be polynucleate and the
remaining isolates were binucleates. According to morphological features of isolates, they were classified into 12 groups.
Polynucleate isolates were identified as Rhizoctonia solani (Kühn). The most aggressive isolate of R. solani was identified
according to sequences of ITS1-5.8S rDNA-ITS4 and the sequence was compared with Thanatephorus cucumeris
(teleomorphic phase) and other R. solani (NCBI GenBank). Sequence and comparison revealed that this isolate is R. solani
AG4-HGI. Anastomosis test carried out between molecular identified isolate and 11 randomly chosen isolates resembles all
groups of polynucleate R. solani. All tested isolates were completely fused between each other indicating that the prevalent AG
of R. solani on faba bean is AG4-HG1.
Other data
| Title | Phenotypic diversity and molecular identification of the most prevalent anastomosis group of Rhizoctonia solani isolated from diseased faba bean plants | Authors | Mohamed Maha Helmy; Gado Emad; Helmy, Maha ; El-Deeb Samir | Keywords | Anastmosis Group, Rhizoctonia Solani AG4-HGI, PCR, Grouping of isolates, Phylogenetic Analysis | Issue Date | 12-Feb-2015 | Publisher | American Journal of Life Sciences | DOI | 10.11648/j.ajls.20150303.24 |
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