Imidacloprid effects on acetylcholinesterase and nicotinic acetylcholine receptor in Apis mellifera. Experimental and molecular modeling approaches
Hussein M. Ali; Basma Abdel-Aty; Walaa El‑Sayed; Faiza M. Mariy; Gamal M. Hegazy;
Abstract
Although the neonicotinoid insecticides have good selectivity towards insects rather than vertebrates, they have
severe effects on honeybee production and pollination activities. Therefore, the effects of imidacloprid (IMI), the
most used neonicotinoid, on the two main bioreceptors, acetylcholinesterase (AChE) and nicotinic acetylcholine
receptor alpha subunit (nAChRα1) of honeybees were examined to identify their roles in honeybee toxicity and
possible binding sites which assist in selecting and designing neonicotinoids. In vivo, IMI showed a high inhib-
itory effect on AChE (IC50 5.63 mg/L); however, the effect was much lower in vitro experiment (IC50 719 mg/L).
This result induced us to examine the IMI effect on AChE gene expression which revealed that the AChE-2 gene
severe effects on honeybee production and pollination activities. Therefore, the effects of imidacloprid (IMI), the
most used neonicotinoid, on the two main bioreceptors, acetylcholinesterase (AChE) and nicotinic acetylcholine
receptor alpha subunit (nAChRα1) of honeybees were examined to identify their roles in honeybee toxicity and
possible binding sites which assist in selecting and designing neonicotinoids. In vivo, IMI showed a high inhib-
itory effect on AChE (IC50 5.63 mg/L); however, the effect was much lower in vitro experiment (IC50 719 mg/L).
This result induced us to examine the IMI effect on AChE gene expression which revealed that the AChE-2 gene
Other data
| Title | Imidacloprid effects on acetylcholinesterase and nicotinic acetylcholine receptor in Apis mellifera. Experimental and molecular modeling approaches | Authors | Hussein M. Ali; Basma Abdel-Aty ; Walaa El‑Sayed; Faiza M. Mariy; Gamal M. Hegazy | Keywords | Neonicotinoids , nAChR , AChE ,Honeybee | Issue Date | 3-Apr-2024 | Publisher | Chemosphere | DOI | DOI: 10.1016/j.chemosphere.2024.141899 |
Attached Files
| File | Description | Size | Format | Existing users please Login |
|---|---|---|---|---|
| chemosphere 8.8 Q1 .pdf | Although the neonicotinoid insecticides have good selectivity towards insects rather than vertebrates, they have severe effects on honeybee production and pollination activities. Therefore, the effects of imidacloprid (IMI), the most used neonicotinoid, on the two main bioreceptors, acetylcholinesterase (AChE) and nicotinic acetylcholine receptor alpha subunit (nAChRα1) of honeybees were examined to identify their roles in honeybee toxicity and possible binding sites which assist in selecting and designing neonicotinoids. In vivo, IMI showed a high inhib- itory effect on AChE (IC50 5.63 mg/L); however, the effect was much lower in vitro experiment (IC50 719 mg/L). This result induced us to examine the IMI effect on AChE gene expression which revealed that the AChE-2 gene | 5.88 MB | Adobe PDF | Request a copy |
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