Developing transgenic wheat to encounter rusts and powdery mildew by overexpressing barley chi26 gene for fungal resistance
Eissa, Hala F; Hassanien, Sameh E; Ramadan, Ahmed M; El-Shamy, Moustafa M; Saleh, Osama M; Shokry, Ahmed M; Abdelsattar, Mohamed; Morsy, Yasser B; El-Maghraby, Maher A; Alameldin, Hussien F; Hassan, Sabah M; Osman, Gamal H; Mahfouz, Hesham T; Gad El-Karim, Gharib A; madkour, magdy; Bahieldin, Ahmed;
Abstract
The main aim of this study was to improve fungal resistance in bread wheat via transgenesis. Transgenic wheat plants harboring barley chitinase (chi26) gene, driven by maize ubi promoter, were obtained using biolistic bombardment, whereas the herbicide resistance gene, bar, driven by the CaMV 35S promoter was used as a selectable marker.
Other data
| Title | Developing transgenic wheat to encounter rusts and powdery mildew by overexpressing barley chi26 gene for fungal resistance | Authors | Eissa, Hala F; Hassanien, Sameh E; Ramadan, Ahmed M; El-Shamy, Moustafa M; Saleh, Osama M; Shokry, Ahmed M; Abdelsattar, Mohamed; Morsy, Yasser B; El-Maghraby, Maher A; Alameldin, Hussien F; Hassan, Sabah M; Osman, Gamal H; Mahfouz, Hesham T; Gad El-Karim, Gharib A; madkour, magdy ; Bahieldin, Ahmed | Keywords | Chitinase activity; Southern; Substantial equivalence; Transgenesis; qPCR; qRT-PCR | Issue Date | 2017 | Journal | Plant methods | ISSN | 1746-4811 | DOI | 10.1186/s13007-017-0191-5 | PubMed ID | 28539970 | Scopus ID | 2-s2.0-85019959300 |
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