Bio-efficacy of two algae against bruchidius incarnatus, physiological and cytogenetic effects on vicia faba

Abstract

Background and Objective: The bean seed beetle Bruchidius incarnatus is a major insect pest for stored grains that causes great economic damage. The investigated research aimed to evaluate the efficacy of two species of algae: Fucus vesiculosus and Spirulina platensis as natural alternative pesticides against Bruchidius incarnatus stages. Materials and Methods: The efficacy of two tested algae with amounts of 0.25, 0.35 and 0.50 g were evaluated on B. incarnatu stages. The activities of some biochemical components were assayed to determine the algae effect. Seed germination and growth parameters were studied. Results: F. vesiculosus caused higher potent on larval and adult stages than S. platensis. Antioxidant enzymes Glutathione Peroxidase (GPX) and Superoxide Dismutases (SOD) in treated adults have reached the highest level when compared with control. Some biochemical components in adults were affected also by algae treatment. Treatment with two algae caused stimulation of seedling and germination development. On the other hand, both types of algae occurred an expansion in the mitotic index and low levels of abnormalities. Conclusion: Both types of algae are considered a promising Bio-insecticide in controlling stored grain pests and it may be considered Bio-eco-friendly in pest management.