Efficiency of three bio-components against broad bean beetle (Bruchidius incarnates) and their effect on germination, seedling growth and cytogenetic changes of Vicia faba L. plants

Abstract

The effect of Radiant SC 12%, Fucus vesiculosus brown alga and Spirulina platensis cyanobacterium were evaluated on the mortality of adult Bruchidius incarnates. Data investigated significant differences where the percentage mortality by F. vesiculolus (0.25 and 0.50 gm) reached 100% after 14 days and 16 days respectively. Radiant (3.75 ppm) recorded 100 % mortality after 16 days while radiant (0.94 and 1.87 ppm) recorded 93.3% mortality after16 days. S. platensis (0.25gm and 0.50 gm) seem to be less potent against the insect where the percent mortality recorded 60 and 66.6 % after 16 days respectively. Results revealed a pronounced inhibitory effect on seed germination and seedling growth of Vicia faba. plants in infested seeds and radiant treatments; while fucus and spirulina showed a stimulatory effect. Cytogenetic analysis showed inhibition of mitotic activity with increased percentage of chromosomal aberrations in Vicia faba. plants in infested seeds and radiant treatments; both kinds of algae showed increasing of mitotic index and low percentage of abnormalities. The concentration 3.75 ppm of radiant exhibited the lower value of mitotic index (MI=6.7%) and the higher percentage of chromosomal abnormalities (CA=43.28%) compared to control (8.3% and 13.25%) respectively.