CYTOTOXICITY OF IMIDACLOPRID AND MYCLOBUTANIL PESTICIDES ON THREE CANCER CELL LINES
Shaimaa M.S. Mohamed;
Abstract
Three cancer cell lines, i.e. HEpG-2 (human
liver carcinoma), MCF-7 (human breast adenocarcinoma), and PC3 (Prostatic Small Cell Carcinoma) were used to determine the cytotoxic effects
of the neonicotinoid insecticide (imidacloprid) and
conazole fungicide (myclobutanil). Cytotoxicity was
measured by neutral red incorporation (NRI) assay. The lowest concentration of the tested pesticides (0.5 μg/ml) was toxic. With the increase of
the concentration up to 80 μg/ml, the Department
of plant protection, Faculty of Agric., Ain Shams
University, shoubra Elkheima, Cairo, Egypt
Department of Genetics, Faculty of Agric., Ain
Shams University, Cairo, Egypt damage degree of
the cellular form and size was more serious. The
midpoint cytotoxicity value, (NRI50) for imidacloprid
and myclobutanil for HEpG-2, MCF-7, and PC3
cancer cell lines were 110.5, 67.7 and 67.6 μg/ml
and 38.12, 41 and 27.5 μg/ml, respectively. In
general, myclobutanil was very toxic in the three
cancer cell lines compared with imidacloprid.
liver carcinoma), MCF-7 (human breast adenocarcinoma), and PC3 (Prostatic Small Cell Carcinoma) were used to determine the cytotoxic effects
of the neonicotinoid insecticide (imidacloprid) and
conazole fungicide (myclobutanil). Cytotoxicity was
measured by neutral red incorporation (NRI) assay. The lowest concentration of the tested pesticides (0.5 μg/ml) was toxic. With the increase of
the concentration up to 80 μg/ml, the Department
of plant protection, Faculty of Agric., Ain Shams
University, shoubra Elkheima, Cairo, Egypt
Department of Genetics, Faculty of Agric., Ain
Shams University, Cairo, Egypt damage degree of
the cellular form and size was more serious. The
midpoint cytotoxicity value, (NRI50) for imidacloprid
and myclobutanil for HEpG-2, MCF-7, and PC3
cancer cell lines were 110.5, 67.7 and 67.6 μg/ml
and 38.12, 41 and 27.5 μg/ml, respectively. In
general, myclobutanil was very toxic in the three
cancer cell lines compared with imidacloprid.
Other data
| Title | CYTOTOXICITY OF IMIDACLOPRID AND MYCLOBUTANIL PESTICIDES ON THREE CANCER CELL LINES | Authors | Shaimaa M.S. Mohamed | Keywords | Myclobutanil, Imidacloprid, Cell line, Cytotoxicity, NRI assay | Issue Date | 29-May-2017 | Publisher | Arab Univ. J. Agric. Sci. | Volume | 25 | Issue | 2 | Start page | 331 | End page | 338 |
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