Genotoxic effects of Imidacloprid and Myclobutanil on Drosophila Melanogaster

Abstract

The genotoxic effects and carcinogenic activity of the neonicotinoid insecticide (imidacloprid) and conazole fungicide (myclobutanil) were estimated on/in D. melanogaster. In this respect, the acute effects of the two tested pesticides on D. melanogaster adults and their genotoxic effects using the drosophila wts-SMART test based on tumor suppressor gene, warts (wts) were measured. The values of LC50 for imidacloprid and myclobutanil on D.melanogaster adults were 13 and 37 ppm, respectively. Imidacloprid and myclobutanil treatments produced a highly significant increase in frequency of inducing wts clone spots in both males and females (0.183, 0.154/ Imidacloprid and 0.10 and 0.14/myclobutanil, respectively). In addition, the two tested pesticides have evidence of genotoxic potential using SMART assays and showed clearly positive result. In general, imidacloprid show highly genotoxic effects compared with myclobutanil, whereas both pesticides showed high carcinogenic activity and prob ability of potential risk to humans.