Glutathione-S-Transferase Response Towards Imidacloprid in Honeybees (Apis mellifera L.)
Basma Abdel-Aty;
Abstract
Imidacloprid is a systematic neonicotinoid widely used to combat
piercing-sucking insects; however, neonicotinoids, despite having low
effects on vertebrates, showed high adverse effects on honeybees (Apis
mellifera L.). Glutathione-S-transferases (GST) are an important constituent
of the defense system in detoxifying invading chemicals. This work exam-
ines the effects of imidacloprid on GST activity in vivo and in vitro condi-
tions. Results revealed that there are two responses of GST activity toward
IMI treatment; first, stimulation of enzyme activity to combat and detoxify
the insecticide. Second, direct inhibition of GST, which is confirmed by the
in vitro inhibition with IC50 887.42 ppm. At a short exposure time to IMI (2
hours) at an IMI concentration of 0.35 ppm, the enzyme was stimulated up
to 113% while increasing exposure time or IMI concentration, the inhibition
effect dominates.
piercing-sucking insects; however, neonicotinoids, despite having low
effects on vertebrates, showed high adverse effects on honeybees (Apis
mellifera L.). Glutathione-S-transferases (GST) are an important constituent
of the defense system in detoxifying invading chemicals. This work exam-
ines the effects of imidacloprid on GST activity in vivo and in vitro condi-
tions. Results revealed that there are two responses of GST activity toward
IMI treatment; first, stimulation of enzyme activity to combat and detoxify
the insecticide. Second, direct inhibition of GST, which is confirmed by the
in vitro inhibition with IC50 887.42 ppm. At a short exposure time to IMI (2
hours) at an IMI concentration of 0.35 ppm, the enzyme was stimulated up
to 113% while increasing exposure time or IMI concentration, the inhibition
effect dominates.
Other data
| Title | Glutathione-S-Transferase Response Towards Imidacloprid in Honeybees (Apis mellifera L.) | Authors | Basma Abdel-Aty | Keywords | GST, Neonicotinoid, Insecticide, Insect resistance, In vivo, In vitro | Issue Date | 23-Dec-2023 | Publisher | Arab Universities Journal of Agricultural Sciences | DOI | DOI:0000-0002-6524-0780 |
Attached Files
| File | Description | Size | Format | Existing users please Login |
|---|---|---|---|---|
| GST.pdf | Imidacloprid is a systematic neonicotinoid widely used to combat piercing-sucking insects; however, neonicotinoids, despite having low effects on vertebrates, showed high adverse effects on honeybees (Apis mellifera L.). Glutathione-S-transferases (GST) are an important constituent of the defense system in detoxifying invading chemicals. This work exam- ines the effects of imidacloprid on GST activity in vivo and in vitro condi- tions. Results revealed that there are two responses of GST activity toward IMI treatment; first, stimulation of enzyme activity to combat and detoxify the insecticide. Second, direct inhibition of GST, which is confirmed by the in vitro inhibition with IC50 887.42 ppm. At a short exposure time to IMI (2 hours) at an IMI concentration of 0.35 ppm, the enzyme was stimulated up to 113% while increasing exposure time or IMI concentration, the inhibition effect dominates. | 179.63 kB | Adobe PDF | Request a copy |
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