Advancement in Wound healing potential by the Application of Folate functionalized Metallic Colloidal Antibiotherapy.

Abstract

Folic Acid (FA) is introducing itself as a useful addition to wound management protocol. Herein, we report a novel eco-friendly method to prepare silver nanoparticles (AgNPs) using the anabolic FA as a reducing agent. The synthesis of FA-stabilized AgNPs (FA-AgNPs) was confirmed by spectrophotometric analysis, revealing the presence of the characteristic absorption peak corresponding to the Surface Plasmon Resonance of colloidal FA-AgNPs. FA intercalation in the colloidal AgNPs was proved by EDX, FTIR and UV spectrophotometry. FA-AgNPs loaded with cefotaxime sodium (cef.Na) demonstrated a synergistic antibacterial activity against both gram-positive and gram-negative. Superior cell survival, using normal skin fibroblasts, was proved in the presence of FA, in addition to enhanced cell survival at high Ag content due to the large-sized particles obtained. The wound healing activity, evaluated in injured rats, showed more than 85% of wound closure after 2 weeks. As healing proceeds, the oxidative stress decreases as delineated by a decrease in Malondialdehyde (MDA) and an increase in Superoxide Dismutase (SOD) levels.

Statement of significance: This study focuses of synthesizing silver nanoparticles in an eco-friendly way using anabolic folic acid, then loading it with a broad spectrum antibiotic, cefotaxime sodium. This environmentally safe green method produced colloidal nanoparticles combining the synergistic antibacterial potential of silver and the antibiotic, producing low mutagenic risk potion, considered an illuminated hope to control and prevent microbial resistance. Folic acid being engulfed in the silver nanoparticles decorated with cefotaxime sodium produced a mosaic working in harmony to achieve clean rapid and scar-less wound healing in short time frame. Such combination could be easily loaded on biogenic wound dressings’ platform to facilitate its application on wounded surface.