Evaluation of the Apoptotic Effect of Punicalagin on Tongue Carcinoma Cell (In-vitro study)

Abstract

Background: Squamous cell carcinoma most frequently develops in the tongue in the oral cavity (SCC), (SCC). Due to increased local invasion, a high likelihood of recurrence, and metastasis to the cervical lymph node, tongue cancer has a poor prognosis. It's been demonstrated that the pomegranate compound punicalagin (PUN) causes amazing biological reactions. The aim of this study was to assess the cytotoxic effect of punicalagin. Methods: This was in-vitro study that was done on Human tongue squamous cell carcinoma cell line which supplied from the Cell Culture Department – Nawah Scientific Research Center, Cairo, Egypt at Central Lab of Stem Cells, and Biomaterial Applied Research (CLSBAR) in Faculty of Dentistry, Ain Shams University. we assess cytotoxicity of punicalagin supplied from “Sigma Aldrich, Germany through MMT assay, evaluation of apoptosis by annexin-v/Propidium iodide using flowcytometry method and test the underlying apoptotic effect of punicalagin on tongue cancer cells as one of the major anti-tumor effects. Results: The half maximal cytotoxic outcome of Punicalagin was (185 µmol) concentration. In test group treated with (185µmol/ml) of punicalagin the percentage of apoptotic cell, necrotic cells and live cells was (51.3% ,2.3%, 46.7%) respectively Conclusion: Punicalagin has a cytotoxic effect on Human tongue squamous cell carcinoma cell line and considered as valued anticancer agent