NUCLEAR AREA FACTOR FOR ESTIMATION OF APOPTOSIS IN GRAVIOLA-TREATED SQUAMOUS CELL CARCINOMA CELL LINE. (EX-VIVO STUDY).
HM Baghdadi; SM Masloub Ali; Ghada Alaa Mohamed Aziz Ibrahim;
Abstract
Aim and Objectives: This work is conducted to estimate the apoptotic effect of Graviola on tongue
squamous cell carcinoma cell line by calculating the nuclear area factor (NAF) of the Graviola-treated cells.
Material and Methods: Tongue squamous cell carcinoma cell line (HNO-97) was used in the current work.
The drug tested in the current work was Graviola (Annona Muricata). The cytotoxicity of Graviola on cultured
HNO-97 cells was evaluated by using the MTT cytotoxicity assay. Software for image analysis was used to
estimate the NAF of Graviola-treated cells at every concentration. Obtained data were then statistically analyzed.
Results: The obtained data showed a decrease in the NAF mean values of HNO-97 cells which treated with
various concentrations of Graviola at various durations as compared to untreated cells.
Conclusion: The current data indicated that estimation of NAF is a sensitive technique for prediction of early
apoptosis of Graviola-treated squamous cell carcinoma cell line.
squamous cell carcinoma cell line by calculating the nuclear area factor (NAF) of the Graviola-treated cells.
Material and Methods: Tongue squamous cell carcinoma cell line (HNO-97) was used in the current work.
The drug tested in the current work was Graviola (Annona Muricata). The cytotoxicity of Graviola on cultured
HNO-97 cells was evaluated by using the MTT cytotoxicity assay. Software for image analysis was used to
estimate the NAF of Graviola-treated cells at every concentration. Obtained data were then statistically analyzed.
Results: The obtained data showed a decrease in the NAF mean values of HNO-97 cells which treated with
various concentrations of Graviola at various durations as compared to untreated cells.
Conclusion: The current data indicated that estimation of NAF is a sensitive technique for prediction of early
apoptosis of Graviola-treated squamous cell carcinoma cell line.
Other data
| Title | NUCLEAR AREA FACTOR FOR ESTIMATION OF APOPTOSIS IN GRAVIOLA-TREATED SQUAMOUS CELL CARCINOMA CELL LINE. (EX-VIVO STUDY). | Authors | HM Baghdadi; SM Masloub Ali; Ghada Alaa Mohamed Aziz Ibrahim | Keywords | Graviola, Nuclear Area Factor, MTT assay | Issue Date | Jun-2021 | Publisher | Ain Shams University, Faculty of Dentistry | Journal | Ain Shams Dental Journal | Volume | 22 | Issue | 2 | Start page | 77 | End page | 83 | DOI | https://doi.org/10.21608/asdj.2021.75784.1045 |
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