Transplanted adipose derived mesenchymal stem cells attenuate the acute renal injury induced by cisplatin in rats
Sakr, A.E.I.; Elhai, W.M.A.; Ali, H.E.-S.; Abo Zeid, Asmaa Abdelmonem;
Abstract
Background: Traditional therapeutic strategies used for the treatment of acute kidney injury (AKI) proved to be less effective
in reducing the morbidity and mortality rate. Recently, stem cell therapy showed a promise for treatment of this complex
disorder.
Aim: To investigate the therapeutic role of adipose-derived mesenchymal stem cells (AD-MSCs) in treatment of cisplatininduced
nephrotoxicity as a model of AKI.
Material and Methods: Twenty adult female Wister rats were divided equally into four groups. Group I was the control, the
other three groups received a single intraperitoneal injection of cisplatin (5 mg /kg), where group II were sacrificed after one
day from cisplatin injection, group III were sacrificed after seven days from cisplatin injection and group IV received adult
male rat AD-MSCs (2x106 cells/rat) in tail vein one day after cisplatin injection and were sacrificed seven days after cisplatin
injection.
Results: The histopathological changes in the renal cortex were more obviously detected in group III than in group II. These
changes include congested and shrunken glomeruli, dilated Bowman's space and loss of proximal convoluted tubules brush
borders. Moreover, distal tubular cells showed cytoplasmic vacuolization, with pyknotic nuclei and presence of intraluminal
hyaline casts. Interstitial collagen deposition was also noticed. In group IV, AD-MSCs administration almost restores the renal
histological architecture. Increased tubular cell proliferation with marked reduction of the interstitial inflammation and fibrosis
were also detected. However, some renal glomeruli and tubules showed degenerative changes. Male rat derived-stem cells
were detected in the female kidney tissue by Y chromosome PCR technique.
Conclusion: Administration of AD-MSCs had a potential regenerative effect for the management of AKI.
Keywords: Acute kidney injury, adipose-derived mesenchymal stem cells, cisplatin, Y chromosome
in reducing the morbidity and mortality rate. Recently, stem cell therapy showed a promise for treatment of this complex
disorder.
Aim: To investigate the therapeutic role of adipose-derived mesenchymal stem cells (AD-MSCs) in treatment of cisplatininduced
nephrotoxicity as a model of AKI.
Material and Methods: Twenty adult female Wister rats were divided equally into four groups. Group I was the control, the
other three groups received a single intraperitoneal injection of cisplatin (5 mg /kg), where group II were sacrificed after one
day from cisplatin injection, group III were sacrificed after seven days from cisplatin injection and group IV received adult
male rat AD-MSCs (2x106 cells/rat) in tail vein one day after cisplatin injection and were sacrificed seven days after cisplatin
injection.
Results: The histopathological changes in the renal cortex were more obviously detected in group III than in group II. These
changes include congested and shrunken glomeruli, dilated Bowman's space and loss of proximal convoluted tubules brush
borders. Moreover, distal tubular cells showed cytoplasmic vacuolization, with pyknotic nuclei and presence of intraluminal
hyaline casts. Interstitial collagen deposition was also noticed. In group IV, AD-MSCs administration almost restores the renal
histological architecture. Increased tubular cell proliferation with marked reduction of the interstitial inflammation and fibrosis
were also detected. However, some renal glomeruli and tubules showed degenerative changes. Male rat derived-stem cells
were detected in the female kidney tissue by Y chromosome PCR technique.
Conclusion: Administration of AD-MSCs had a potential regenerative effect for the management of AKI.
Keywords: Acute kidney injury, adipose-derived mesenchymal stem cells, cisplatin, Y chromosome
Other data
| Title | Transplanted adipose derived mesenchymal stem cells attenuate the acute renal injury induced by cisplatin in rats | Authors | Sakr, A.E.I.; Elhai, W.M.A.; Ali, H.E.-S.; Abo Zeid, Asmaa Abdelmonem | Issue Date | 2017 | Journal | Egyptian Journal of Histology | Volume | 40 | Issue | 2 | Start page | 169 | End page | 183 | ISSN | 2090-2417 | DOI | 10.21608/EJH.2017.4075 | Scopus ID | 2-s2.0-85055717524 |
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