The assessment of some enzymes in human body by new optical sensors

Abstract

Fluorescence spectroscopy has many advantages making it an appealing choice for low concentrationssamples analysis.Fluorometry may achieve limits of detection several orders of magnitude lower than those of most other techniques. This is known as the fluorescence advantage .fluorescence measurements are rapid and inexpensive, Because of the low detection limits, so fluorescence is widely used for quantification of trace constituents of biological and environmental samples.

The study is divided into three chapters:

Chapter one Introduction and literature review: This Chapter includes a general introduction on fluorescence spectroscopy and its advantage in modern analytical techniques and its theory about sensing and different types of fluorescent probes. A literature review on the measurement of enzymes such as glucose oxidase and xanthine oxidaseusing different method of detection.Recent references related to research has been used.

Chapter two Describes a highly selective and sensitive spectrofluorimetric method for the assessment of xanthine oxidase enzyme (XO) in Serum Samples based on the quenching of luminescence intensity of the Optical sensor 2- [chloro-Bis- (triphenylphosphine) palladium(II)] – 6 – methoxy – 3 - quinolinecraboxaldehydes [Pd-BQC]. It was found that there is energy transfer between photo probe and uric acid (which result from react xanthine + H2O + O2 in presence xanthine oxidase enzyme) in addition to measurement of photo probe emission at exication wave length (λEX =420 nm) under different condition of solvent and pH. The optimum conditions for measurement are DMSO as solvent and pH=8.9.photo probe has been used for uric acid concentrations by quenching of the photo probe(Pd-BQC) band at wave length of emission(λem=530 nm) after adding different concentration of uric acid fluorescence intensity under optimum condition and making linear relation between fluorescence intensity and 1/different concentration and making wide linear working range (4.2 x 10−9 to 3.1 ×10−6mol L-1) [uric acid] with a correlation coefficient of 0.995 and a detection limit of 7.9 × 10−9mol L-1. Thus,the use ofthephoto probein theenzymexanthineoxidaseactivity estimateby measuringand estimatinguricacid indifferent samplesofserumtothe many ofLiver diseasepatients.

Chapter three Describes a highly selective and sensitive spectrofluorimetric method for the assessment of glucose oxidase [GO] in Serum Samples based on the quenching of luminescence intensity of the Optical sensor 2-(2,2-dichloro-3-oxoindolin-1-yl)-3H-indol-3-one (DOI). It was found that there is energy transfer between photo probe and H2O2 (which result from react β-D glucose + H2O + O2 in presence glucose oxidase enzyme) in addition to measurement of photo probe emission at exication wave length (λex =410 nm) under different condition of solvent and pH. Theoptimum conditions for measurement are acetonitrile as solvent and pH=8.9. photo probe has been used for uric acid concentrations by quenching of the photo probe(DOI) band at wave length of emission(λem=520 nm) after adding different concentration of uric acid fluorescence intensity under optimum condition and making linear relation between fluorescence intensity and 1/different concentration and making wide linear working range (5.0 × 10−7 to 2.0 ×10−9mol L−1 (r = 0.998). And The detection limit (S:N = 3) (LOD) is 2.9 x 10-9mol L−1 .. Thus,the use ofthephoto probein theenzymeglucoseoxidaseactivity estimatebymeasuringand estimatingH2O2indifferent samplesofserumtothe many diabetespatients.