Flow Cytometric Assessment of CD30 Expression in Adult Patients with Acute Leukemia

Abstract

Acute lymphoblastic leukemia (ALL) is a heterogeneous group of disorders that result from the clonal proliferation and expansion of malignant lymphoid cell in the bone marrow, blood and other organs. It is the most common type of cancer in children and adolescents accounting for 23% to 25% of all malignant diseases. Acute myeloid leukemia (AML) is heterogeneous group of neoplasms that affect hematopoietic cells responsible for the production of myeloid lineages in bone marrow is the second most common type of leukemia among adults with an age-adjusted incidence of 3.6 per 100,000 per year and a median age of 69 years at diagnosis. The objective of the current study was to assess CD30 expression of bone aspiration and/or peripheral blood by flow cytometry in patients with acute myeloid leukemia and acute lymphoblastic leukemia either T cell or B cells type either newly diagnosed or refractory / relapsed cases to evaluate the benefit of anti CD30 in these groups. Our study was carried out on 10 new cases of AML patients and 10 new cases of ALL, 15 refractory / or relapsed cases of AML and15 refractory / or relapsed cases of ALL either T cell or B cells type. Cases was collected from Hematology Unit in Ain Shams University Hospital. Immunophenotypic analysis by flow cytometry of bo