Studying the Role of Thioredoxin-1 Gene Expression in Patients with Acute Leukemia

Amany Mohamed Kamal Shams El Dine;

Abstract


The AL is a heterogeneous group of neoplasms that affect hemopoietic stem cells. They are broadly classified into acute myeloid leukemia (AML) and acute lymphoid leukemia (ALL) based on the cell of origin.AML and adult-onset ALL are aggressive diseases with generally poor prognoses. Leukemia accounts for some 400000 newcases each year (2.8% of all new cancer cases) and265000 deaths. This rather high ratio of deaths /cases (66%) reflects the poor prognosis of leukemia in many parts of the world.
One of the major contributors to malignant transformation in AL is the accumulation of oxidative DNA damage through disrepair or incomplete repair of DNA. Oxidative stress is defined as the bio-molecular damage caused by attack of reactive oxygen species (ROS) upon the cellular constituents of living organisms. The cells and DNA of the human body are constantly exposed to oxidative attacks. During these attacks, although not in all cases, the primary damage is being induced by ROS.
Recent progress in the use of DNA microarrays has identified genes that regulate cell cycle control, DNA repair, and apoptosis, which may also participate in disordered cell proliferation and cancer progression in leukemia. Alterations in the basal level of expression of these genes may also affect the drug response and clinical outcome of leukemic patients. The identification of such new gene markers is therefore important not only to gain a basic understanding of the signaling pathways that operate in leukemogenesis, but also to implement enhancements to disease classification systems and to productively target disease with novel therapies.

Therefore, the current study was designed to evaluate the expression of thioredoxin-1 (TXN), one of the major antioxidant systems in the human cell, in adult AML and ALL to investigate a possible relation between TXN expression and oxidative DNA damage and thus determine the potential role of TXN expression in the pathogenesis and treatment outcome of AL. We claim that this is the first study investigating the relation between TXN fold expression and oxidative DNA damage in AL.
In order to fulfill our aim, this study was conducted on 75 subjects divided into the following three age and sex matched groups:
a) Control group: consisted of 20 healthy adult volunteers (10 males and 10 females).
b) ALL group: consisted of 25 patients (15 males and 10 females).
c) AML group: consisted of 30 patients (15 males and 15 females).
Complete medical history was taken from all subjects and periferal blood (PB) samples (8-10 ml) were collected from all subjects for the determination of:
1- TXN fold expression.
2- Serum 8-OHdG.
3- DNA strand breaks.
Then statistical analysis was done for these parameters to gain more insight into our results.


Other data

Title Studying the Role of Thioredoxin-1 Gene Expression in Patients with Acute Leukemia
Other Titles دراسة دور اظهار تاثيرات جين الثيوريدوكسين-1 فيمرضى سرطان الدم الحاد
Authors Amany Mohamed Kamal Shams El Dine
Issue Date 2016

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