Cyclooxygenase 2 and prostaglandin E2 are not involved in N-nitrosodiethylamine-initiated early rat hepatocarcinogenesis
Said, Mahmoud Mohamed;
Abstract
The present study was undertaken to investigate the effect of dietary supplementation with nimesulide or eugenol on N-nitrosodiethylamine (DEN)-initiated early hepatocarcinogenesis in F344 male rats. Both compounds did not alter the expression of cytochrome P450 (CYP) 2E1, the enzyme that plays a major role in the activation of DEN to genotoxic products; however, nimesulide induced the expression of CYP1A1. Western blot analysis revealed that COX-1 and COX-2 protein expressions were not modulated by DEN compared with normal controls. Furthermore, postinitiation feeding with nimesulide or eugenol did not modulate COX-2 protein expression in normal or DEN-treated rats, whereas eugenol significantly increased the liver prostaglandin E2(PGE2) levels of DEN-injected animals compared with the DEN controls. Ultimately, nimesulide or eugenol did not modify DEN-induced hepatocarcinogenesis as evidenced by insignificant changes in the number and size of preneoplastic placental glutathione S-transferase (GST-P) positive liver foci compared with the DEN controls. These results suggest that COX-2, as well as prostaglandin E2, may play no role in the post-initiation development of DEN-induced rat hepatocarcinogenesis at an early stage.
Other data
Title | Cyclooxygenase 2 and prostaglandin E2 are not involved in N-nitrosodiethylamine-initiated early rat hepatocarcinogenesis | Authors | Said, Mahmoud Mohamed | Issue Date | 1-Dec-2009 | Journal | Journal of Toxicologic Pathology | DOI | 4 https://api.elsevier.com/content/abstract/scopus_id/75749116280 263 22 10.1293/tox.22.263 |
Scopus ID | 2-s2.0-75749116280 |
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