ANTIMICROBIAL AND ANTIPROLIFERATIVE, PRO-APOPTOTIC ACTIONS OF KOMBUCHA FERMENTED SOLUTIONS AGAINST COLON AND HEPTAO CANCER CELL LINES

Abstract

Introduction: Kombucha fermented solutions (KFSs) were produced from tea (KTFS), rice (KRFS) and barely (KBFS) after 8 and 10 days. These solutions tested as antimicrobial activity against microorganisms (Listeria monocytogenes, Shigelladysenteriae, Salmonella enteritidis, Staphylococcus aureus, Citrobacter sp., Klebsiella pneumoniae, Esherichia coli and Candida albicans) by agar well diffusion assay. KTFS was higher inhibitory activity than KRFS or KBFS, which gave the largest clear halo-zone diameter against S. enteritidis. Minimum lethal concentration: minimum inhibition concentration ratio wascalculated to known the KFSs action. All tested KFSs were micobicidal effect against S. aureus and C. albicans (≤2) and microbiostatic effect against other pathogenic bacteria (≥4). Objective: The study extends to examine the growth inhibitory effects and Apoptotic abilities of KFSS, on human colon (HCT-116) and liver (HEpG-2) cancer cells. Material and method: using Neutral Red Uptake Assay and the AO/EB dual Staining assays to detect anti proliferative and apoptosis properties. Results: indicated that KTFS had more antitumor activity against different tumor cells than KRFS and KBFS. Although all tested KFSs were found to reduce the cell viability in a concentration manner, but the magnitude of reduction was high in case of KTFS with lower IC50 values, and induces a higher percentage of apoptotic cells after 24 h of exposure of HEpG-2 cell line. These results suggest that the KFSs possess interesting antiproliferative properties and induce apoptosis on the HCT-116 and HEpG-2 cancer cells associated with significant antimicrobial activity. These findings provide additional support for the traditional use of KFSs in the treatment of metabolic diseases and various types of cancer.