Antibacterial, antibiofilm and antitumor activities of grape and mulberry leaves ethanolic extracts towards bacterial clinical strains
Elshahat M. Ramadana; Abou-Taleb, Khadiga; Gehan F. Galala; Nermin S. Abdel-Hamid;
Abstract
The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were measured at concentrations of 0.01–2.56 mg/mL of grape and mulberry leaves ethanolic extracts. The MIC values were ranged from 0.08 to 0.16 mg/mL against Ps. aeruginosa Ps9, and 0.32 mg/mL against each of S. aureus St3, E. coli Ec3, and S. typhi Sa1. Whereas, the MBC values were ranged from 0.32 to 1.28 mg/mL of the tested extracts. The effects of the tested extracts were also studied representing the bactericidal effect of the grape extract with a ratio of 2 against all investigated isolates, except S. typhi Sa1. Whereas, the mulberry extract had a bactericidal effect towards S. aureus St3 and E. coli Ec3 with ratio of 2, and a bacteriostatic effect against Ps. aeruginosa Ps9 and S. typhi Sa1 with a ratio ≥4. The investigated bacteria found to have a strong ability to form biofilms with
densities ranged from 0.67 to 0.80. Both tested extracts inhibited these biofilms with percentages ranged from 48 to 66% at sub-inhibitory concentrations (SICs) ranged from 0.04 to 0.16 mg/mL. In addition, the tested extracts have an excellent cytotoxic activity towards colon cancer cell lines (HCT-16). Five phenolic compounds detected in the tested extracts of grape and mulberry using high performance liquid chromatography (HPLC) after 9.53 min of the retention time. The phenolic compounds of both tested extracts were gallic, coumaric, ferulic, chlorogenic and caffeic with concentrations ranged from 1.28 to 6.56 µg/mL
densities ranged from 0.67 to 0.80. Both tested extracts inhibited these biofilms with percentages ranged from 48 to 66% at sub-inhibitory concentrations (SICs) ranged from 0.04 to 0.16 mg/mL. In addition, the tested extracts have an excellent cytotoxic activity towards colon cancer cell lines (HCT-16). Five phenolic compounds detected in the tested extracts of grape and mulberry using high performance liquid chromatography (HPLC) after 9.53 min of the retention time. The phenolic compounds of both tested extracts were gallic, coumaric, ferulic, chlorogenic and caffeic with concentrations ranged from 1.28 to 6.56 µg/mL
Other data
Title | Antibacterial, antibiofilm and antitumor activities of grape and mulberry leaves ethanolic extracts towards bacterial clinical strains | Authors | Elshahat M. Ramadana; Abou-Taleb, Khadiga ; Gehan F. Galala; Nermin S. Abdel-Hamid | Keywords | Vitis vinifera Morus alba Pathogenic bacteria Minimum inhibitory concentration Minimum bactericidal concentration Antitumor activity HCT-16 cell line Phenolic acids | Issue Date | 8-Dec-2017 | Journal | Annals of Agricultural Sciences | Volume | 62 | Start page | 151 | End page | 159 | DOI | doi.org/10.1016/j.aoas.2017.11.002 |
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