Nutritional and Environmental Factors Affecting Cellulase Production by Two Strains of Cellulolytic Bacilli

Abou-Taleb, Khadiga A.A., Mashhoor, W.A., Nasr, Sohair A.,Sharaf, M.S. and Abdel-Azeem, Hoda H.M. ; Abou-Taleb, Khadiga 


Abstract


Effect of some nutritional and environmental factors on growth and cellulase production by Bacillus alcalophilus S39 and Bacillus amyloliquefaciens C23 was investigated. Results indicated that 1% carboxymethylcellulose (CMC) and 0.7% yeast extract were most effective as the carbon and nitrogen sources respectively. Initial pH 7 and 3% inoculum size found to be optimal for growth and cellulase production. Incubation temperature at 30 and 45ºC achieved the highest activity of cellulase for Bacillus alcalophilus S39 and Bacillus amyloliquefaciens C23 respectively, and the suitable shaking rate was 150 and 200 rpm.


Other data

Keywords Nut ritional & environmental factors, Cellulas e production, B. alcalophilus S39, B. amyloliquefaciens C23.
Issue Date 2009
Publisher INSInet
Source Abou-Taleb, Kh. A.A., Mashhoor, W.A., Nasr, S. A., Sharaf, M.S. and Abdel-Azeem, H. H.M. (2009). Nutritional and Environmental Factors Affecting Cellulase Production by Two Strains of Cellulolytic Bacilli. Australian Journal of Basic and Applied Sciences, 3(3): 2429-2436
Journal Aus tralian Journal of Bas ic and Applied Sciences 
Description Cellulase production by bacteria
URI http://research.asu.edu.eg/handle/123456789/1830


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