PRODUCTION OF VITAMIN B12 BY PROPIONIBACTERIUM FREUDENREICHII AND BACILLUS MEGATERIUM
Abou-Taleb, Khadiga; M. S. Sharaf; W. A. Mashhoor; Sohair, A. Nasr;
Abstract
Effect of some nutritional factors on growth and vitamin B12 production by
Propionibacterium freudenreichii subsp. freudenreichii CCM 1857, and
Propionibacterium freudenreichii subsp. shermanii P1NRC, Bacillus megaterium
DSM2894, and Bacillus megaterium 1066 was investigated. Results indicated that
sodium lactate was the best carbon source for Propionibacterium spp., while glucose
was the best carbon source for B. megaterium. Yeast extract plus ammonium
sulphate was superior nitrogen source for Propionibacterium spp., and yeast extract
was the best nitrogen source for B. megaterium. Use of combination of some
elements (CoCl2 + CuSO4 + MnSO4 + ZnSO4 + MgSO4 + FeSO4) enhanced growth of
Propionibacterium spp. and B. megaterium. Cobalt concentration 10mg l-1 was favor
for Propionibacterium spp. however Cobalt concentration 15mg l-1 was favor for B.
megaterium. High test molasses and corn meal were found to be the best agriculture
by-products for Propionibacterium freudenreichii subsp. freudenreichii CCM 1857 &
Bacillus megaterium DSM 2894 after being added to the basal medium.
Propionibacterium freudenreichii subsp. freudenreichii CCM 1857, and
Propionibacterium freudenreichii subsp. shermanii P1NRC, Bacillus megaterium
DSM2894, and Bacillus megaterium 1066 was investigated. Results indicated that
sodium lactate was the best carbon source for Propionibacterium spp., while glucose
was the best carbon source for B. megaterium. Yeast extract plus ammonium
sulphate was superior nitrogen source for Propionibacterium spp., and yeast extract
was the best nitrogen source for B. megaterium. Use of combination of some
elements (CoCl2 + CuSO4 + MnSO4 + ZnSO4 + MgSO4 + FeSO4) enhanced growth of
Propionibacterium spp. and B. megaterium. Cobalt concentration 10mg l-1 was favor
for Propionibacterium spp. however Cobalt concentration 15mg l-1 was favor for B.
megaterium. High test molasses and corn meal were found to be the best agriculture
by-products for Propionibacterium freudenreichii subsp. freudenreichii CCM 1857 &
Bacillus megaterium DSM 2894 after being added to the basal medium.
Other data
Title | PRODUCTION OF VITAMIN B12 BY PROPIONIBACTERIUM FREUDENREICHII AND BACILLUS MEGATERIUM | Authors | Abou-Taleb, Khadiga ; M. S. Sharaf; W. A. Mashhoor; Sohair, A. Nasr | Keywords | Vitamin B12 production;Agricultural by-products;Microelements;Propionibacterium freudenreichii;Bacillus megaterium. | Issue Date | 2005 | Journal | J. Agric. Sci. Mansoura Univ. | Volume | 30 | Issue | 7 | Start page | 4149 | End page | 4162 |
Attached Files
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