EFFECT OF ENVIRONMENTAL FACTORS ON VITAMIN B12 PRODUCTION BY PROPIONIBACTERIUM FREUDENREICHII AND BACILLUS MEGATERIUM
Abou-Taleb, Khadiga; W. A. Mashhoor; Sohair, A. Nasr; M. S. Sharaf;
Abstract
Effect of some environmental factors on vitaminB12 production
by Propionibacterium freudenreichii subsp. freudenreichii CCM 1857,
and Propionibacterium freudenreichii subsp. shermanii P1NRC,
Bacillus megaterium DSM2894, and Bacillus megaterium 1066 was
investigated. Results indicated that that pH 6.6 to 6.8 were the most
convenient for vitamin B12 by Bacillus megaterium, however the pH
7.0 was the best level for Propionibacterium spp. It was found that
optimum incubation temperature for vitamin B12 production ranged
between 30 - 35ºC. Concerning effect of aeration on biological activity
and vitamin B12 productivity, results indicated that growth under static
culture conditions (4 days) followed by shaking culture (4 days) for
Propionibacterium spp., was the best. While growth under shaking
culture conditions followed by static culture condition and again
followed by shaking culture condition were suitable for Bacillus
megaterium
by Propionibacterium freudenreichii subsp. freudenreichii CCM 1857,
and Propionibacterium freudenreichii subsp. shermanii P1NRC,
Bacillus megaterium DSM2894, and Bacillus megaterium 1066 was
investigated. Results indicated that that pH 6.6 to 6.8 were the most
convenient for vitamin B12 by Bacillus megaterium, however the pH
7.0 was the best level for Propionibacterium spp. It was found that
optimum incubation temperature for vitamin B12 production ranged
between 30 - 35ºC. Concerning effect of aeration on biological activity
and vitamin B12 productivity, results indicated that growth under static
culture conditions (4 days) followed by shaking culture (4 days) for
Propionibacterium spp., was the best. While growth under shaking
culture conditions followed by static culture condition and again
followed by shaking culture condition were suitable for Bacillus
megaterium
Other data
| Title | EFFECT OF ENVIRONMENTAL FACTORS ON VITAMIN B12 PRODUCTION BY PROPIONIBACTERIUM FREUDENREICHII AND BACILLUS MEGATERIUM | Authors | Abou-Taleb, Khadiga ; W. A. Mashhoor; Sohair, A. Nasr; M. S. Sharaf | Keywords | Vitamin B12 production;Cultural conditions;Propionibacterium freudenreichii;Bacillus megaterium. | Issue Date | 2010 | Journal | J. Biol. Chem. Environ. Sci. | Volume | 5 | Issue | 4 | Start page | 509 | End page | 521 |
Attached Files
| File | Description | Size | Format | Existing users please Login |
|---|---|---|---|---|
| VITAMIN B12 (2).pdf | 406.87 kB | Adobe PDF | Request a copy |
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