Essential Oil of Cestrum diurnum L.: GC/MS Analysis, in vitro and in silico Anti-HCoV-229E Effects and Inhibitory Activity against LPS-Induced Inflammation
Bahgat, Dina M; Gad, Haidy; Al-Sayed, Eman Mohamed Kamal; Mahmoud, Sara H; Mostafa, Ahmed; Mahfouz, Nadia M; Eldahshan, Omayma A;
Abstract
Cestrum diurnum L. (Solanaceae) is a fragrant ornamental tree cultivated in different parts around the world. In this study, the essential oil (EO) of the aerial parts was extracted by hydro-distillation (HD), steam distillation (SD) and microwave-assisted hydro-distillation (MAHD). GC/MS analysis of the three EOs revealed that phytol represents the major component in SD-EO and MAHD-EO (40.84 and 40.04%, respectively); while in HD-EO it only represented 15.36%. The SD-EO showed a strong antiviral activity against HCoV-229E with IC50 of 10.93 µg/mL, whereas, MAHD-EO and HD-EO showed a moderate activity with IC50 values of 119.9 and 148.2 µg/mL, respectively. The molecular docking of EO major components: phytol, octadecanol acetate and tricosane showed a strong binding to coronavirus 3-CL(pro). Moreover, the three EOs (50 μg/mL) decreased the levels of NO, IL-6 and TNF-α and suppressed IL-6 and TNF-α gene expression in LPS-induced inflammation model in RAW264.7 macrophage cell line.
Other data
Title | Essential Oil of Cestrum diurnum L.: GC/MS Analysis, in vitro and in silico Anti-HCoV-229E Effects and Inhibitory Activity against LPS-Induced Inflammation | Authors | Bahgat, Dina M; Gad, Haidy ; Al-Sayed, Eman Mohamed Kamal ; Mahmoud, Sara H; Mostafa, Ahmed; Mahfouz, Nadia M; Eldahshan, Omayma A | Keywords | 229E HCoV;Cestrum;Cytokines;Molecular docking;phytol | Issue Date | 22-Feb-2023 | Journal | Chemistry & Biodiversity | ISSN | 1612-1872 1612-1880 |
DOI | 10.1002/cbdv.202201045 | PubMed ID | 36811152 |
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Chemistry Biodiversity - 2023 - Bahgat - Essential Oil of Cestrum diurnum L GC MS Analysis in vitro and in silico.pdf | 6.06 MB | Adobe PDF | Request a copy |
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