Production of L-Asparaginase from some Local Bacterial Strains Using Agro-Industrial Residues and Evaluation of its Anti-tumor Activity

Naglaa, M. Ebeed and Khadiga A. Aboutaleb; Abou-Taleb, Khadiga;

Abstract


L-asparaginase is extracellular enzyme that can be secreted by several microorganisms, making
interesting the biodiversity exploration for searching new microorganisms able to produce this enzyme. Many
agro-industrial residues can be used as potential substrates for production of enzyme. The main objective of this
work was to select a cheap agro-industrial residues for higher L-asparaginase production by four bacterial
strains namely, Acinetobacter radioresistens PS14, Bacillus subtilis SF2, Bacillus marisflavi SO5 and Bacillus
subtilis SW5. The maximum amount of enzyme was obtained during propagation A. radioresistens PS14 and B.
subtilis SW5 on black strap sugar cane molasses as carbon source as well as B. subtilis SF2 and B. marisflavi
SO5 on sesame hulls as a sole carbon source. The addition of by-product such as corn steep liquor was used as
nitrogen source plus L-asparagine gave the highest yield of enzyme in productive medium. A low price
productive medium composed of black strap sugar cane molasses or sesame hulls as carbon source, corn steep
liquor plus L-asparagine as nitrogen source and a mixture of elements were suggested for L-asparaginase
production by tested strains grown in a shake flasks as a batch culture at 30°C for 48 h of fermentation period
expected for A. radioresistens PS14 was grown for 60 h of fermentation period. Moreover, the highest specific
sugar consumption (μs) was noticed during the period of 12-18 h for A. radioresistens PS14 and 6-12 h for B.
subtilis SF2, B. marisflavi SO5 and B. subtilis SW5. Also, specific production rate (μp) of enzyme was obtained
during the period of 6 -12 h for B. subtilis SW5 and 12 - 18 h for A. radioresistens PS14, B. subtilis SF2 and B.
marisflavi SO5.
Screening for carcinogenicity of the L-asparaginase crude extract indicated that the crude enzyme
preparation produced by B. subtilis SF2 and A. radioresistens PS14 strains has evidence of genotoxic potential
using warts-based SMART assays Meanwhile, B. marisflavi SO5 and B. subtilis SW5 strains were safe and
showed clearly negative results.
Accordingly, the results of this study suggested B. marisflavi SO5 and B. subtilis SW5 strains are
promising candidate for producing safely L-asparaginase enzyme that has been used as antitumor agent.


Other data

Title Production of L-Asparaginase from some Local Bacterial Strains Using Agro-Industrial Residues and Evaluation of its Anti-tumor Activity
Authors Naglaa, M. Ebeed and Khadiga A. Aboutaleb ; Abou-Taleb, Khadiga 
Keywords L-asparaginase, agro-industrial residues, Bacillus, leukemia, anti-tumor
Issue Date 2014
Source Ebeed, N. M. and Aboutaleb, Kh. A. (2014). Production of L-Asparaginase from some Local Bacterial Strains Using Agro-Industrial Residues and Evaluation of its Anti-tumor Activity. Current Science International, 9 (1): 53-60.
Journal Current Science International 
ISSN 2077-4435

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