Potential protective effects of epigallocatechin-3-gallate against doxorubicin-induced cardiotoxicity in rats

Abstract

The present study entails the potential cardioprotective effect of EGCG against DOX-induced cardiotoxicity in rats and to clarify the underlying mechanisms via studying its effect on different oxidative stress, inflammatory, apoptotic markers as well as survival signals.

First, determination of the appropriate cardioprotective dose was achieved by constructing dose response study using different doses of EGCG. Animals were divided into five groups: group (I) considered as control received 1ml/kg of distilled water through oral gavage once daily for 12 consecutive days.Group (II) was DOX-treated rats received 1ml/kg of distilled water through oral gavage once daily for 12 consecutive days followed by a single i.p. injection of DOX (15 mg/kg) on the 12th day. Groups (III), (IV) and (V) were EGCG pretreated groups, received 10, 20 and 40 mg/kg of EGCG respectively through oral gavage once daily for 12 consecutive days followed by a single i.p. injection of DOX (15 mg/kg) on the 12th day 1 h after EGCG treatment.Forty-eight hours after DOX injection, rats were anesthetized with ketamine and subjected to ECG recording. Blood samples were collected then animals of all groups were sacrificed, and heart tissues were dissected out for histopathological examination.