"A Study of the Relation between Iron Status and Brain Function in Experimental Animals"
Radwa Wahid Mouhamed Elnagar;
Abstract
The present study was planned to investigate the effect of iron deficiency and iron-load on brain function. Our study included animal and human studies.
(I) The animal study:
Sixty three male weanling albino rats Sprague-Dawely strain of mean body weights 70 ± 10 g were used. The rats were randomly assigned to seven experimental groups. Groups (1 and 2): normal rats which were fed on a control (CN) diet (35mg Fe/Kg diet) for 21 and 28 days, respectively; groups (3 and 4): iron-deficient rats which were fed on an iron-deficient (ID) diet (3mg Fe/ Kg diet) for 21 and 28 days, respectively; group (5): iron-repletion rats which were fed on an iron-deficient diet for three weeks followed by an iron-adequate diet for one week; groups (6 and 7): iron load rats which were fed on an iron-load (IL) diet (250 mg Fe/ Kg diet) for 21 and 28 days, respectively. Hematological variables (hemoglobin, hematocrit and red blood cells count) were estimated in whole blood, as well as blood indices: mean corpuscular hemoglobin (MCH), mean corpuscular volume (MCV) and mean corpuscular hemoglobin concentration (MCHC) were calculated. Serum and brain iron, TIBC, ferritin, transferrin and transferrin saturation were estimated as well as dopamine and serotonin concentration in the brains of different groups.
1- Iron deficient diets for 21 days caused significant decrease in hemoglobin concentration and hematocrit by -12.69% and -13.30%, respectively and non-significant decrease in red blood cells count, as compared to control normal rats for 21 days. The hemoglobin concentration, hematocrit and red blood cells count increased significantly in the iron-repleted group (Repl.) as compared to the normal animals (CN, 28 days) or that stayed on iron deficient diets for 28 days (ID, 28 days).
2- Iron-load rats (28 days) showed non-significant change in hemoglobin, hematocrit and red blood cells count as compared to control normal group (28 days).
3- The values of MCH and MCV of rats fed on iron deficient diets for 28 days were significantly lower by -13.95% and -14.03%, respectively than control normal values recorded at 28 days. Whereas, the MCHC level was not significantly affected following iron deficiency for 28 days from the control groups (CN, 21 and 28 days) and iron-deficient group for 21 days.
4- The present results demonstrate that, iron repletion for one week (Repl.) non-significantly elevated MCH and MCV and non-significantly reduced MCHC as compared with iron-deficient group (ID, 28 days).
5- MCV was markedly elevated in iron-load fed rats (for 21 and 28 days) as compared to control normal groups (for 21 and 28 days).
6- Iron deficiency for both 21 and 28 days showed significant decrease in serum iron and ferritin and increase in TIBC as compared with control groups (CN, 21and 28 days). Whereas, iron-load group showed opposite results.
7- Consuming iron adequate diets for one week after three weeks of iron deficiency, induced significant elevation in the level of serum iron and ferritin and decrease in
(I) The animal study:
Sixty three male weanling albino rats Sprague-Dawely strain of mean body weights 70 ± 10 g were used. The rats were randomly assigned to seven experimental groups. Groups (1 and 2): normal rats which were fed on a control (CN) diet (35mg Fe/Kg diet) for 21 and 28 days, respectively; groups (3 and 4): iron-deficient rats which were fed on an iron-deficient (ID) diet (3mg Fe/ Kg diet) for 21 and 28 days, respectively; group (5): iron-repletion rats which were fed on an iron-deficient diet for three weeks followed by an iron-adequate diet for one week; groups (6 and 7): iron load rats which were fed on an iron-load (IL) diet (250 mg Fe/ Kg diet) for 21 and 28 days, respectively. Hematological variables (hemoglobin, hematocrit and red blood cells count) were estimated in whole blood, as well as blood indices: mean corpuscular hemoglobin (MCH), mean corpuscular volume (MCV) and mean corpuscular hemoglobin concentration (MCHC) were calculated. Serum and brain iron, TIBC, ferritin, transferrin and transferrin saturation were estimated as well as dopamine and serotonin concentration in the brains of different groups.
1- Iron deficient diets for 21 days caused significant decrease in hemoglobin concentration and hematocrit by -12.69% and -13.30%, respectively and non-significant decrease in red blood cells count, as compared to control normal rats for 21 days. The hemoglobin concentration, hematocrit and red blood cells count increased significantly in the iron-repleted group (Repl.) as compared to the normal animals (CN, 28 days) or that stayed on iron deficient diets for 28 days (ID, 28 days).
2- Iron-load rats (28 days) showed non-significant change in hemoglobin, hematocrit and red blood cells count as compared to control normal group (28 days).
3- The values of MCH and MCV of rats fed on iron deficient diets for 28 days were significantly lower by -13.95% and -14.03%, respectively than control normal values recorded at 28 days. Whereas, the MCHC level was not significantly affected following iron deficiency for 28 days from the control groups (CN, 21 and 28 days) and iron-deficient group for 21 days.
4- The present results demonstrate that, iron repletion for one week (Repl.) non-significantly elevated MCH and MCV and non-significantly reduced MCHC as compared with iron-deficient group (ID, 28 days).
5- MCV was markedly elevated in iron-load fed rats (for 21 and 28 days) as compared to control normal groups (for 21 and 28 days).
6- Iron deficiency for both 21 and 28 days showed significant decrease in serum iron and ferritin and increase in TIBC as compared with control groups (CN, 21and 28 days). Whereas, iron-load group showed opposite results.
7- Consuming iron adequate diets for one week after three weeks of iron deficiency, induced significant elevation in the level of serum iron and ferritin and decrease in
Other data
| Title | "A Study of the Relation between Iron Status and Brain Function in Experimental Animals" | Other Titles | دراسة العلاقة بين حالة الحديد و وظيفة المخ في حيوانات التجارب | Authors | Radwa Wahid Mouhamed Elnagar | Issue Date | 2014 |
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