Ultrastructural and Immunohistological Characterization of Cultured Human Corneal Epithelium
Hala M. Fouad El-Mazar;
Abstract
This work was unde1iaken with the aim of studying the histological and immunohistological characteristics of the cultured human corneal
, , epithelial cells. Most of the previous studies that were done to evaluate different corneal events employed animals and animal-derived tissues as an alternative model to human. Morphological, biochemical and species differences had placed such studies in a challenge. The availability of human corneal epithelial cell lines that retain their phenotypic trait and
• were species- as well as tissue-specific might make it possible to model
the human ocular surface in vitro.
I •
'i .'
'
. Cultures of h an corneal epithelial cells were obtained by using two methods of culturing, the explant and the pronease methods. The resulting cells were evaluated histologically and immunohistologically
.. regarding their viability, rate of growth, senility,....etc with the aid of
•phase contrast, transmission electron and indirect immunofluorescence
. .
mtcroscopies.
Materials and Methods:
Eighteen pairs of human donor corneas were obtained from the Michigan eye bank. They were non-reactive for hepatitis B, hepatitis C and AIDS viruses.
The corneas were divided into two groups each included 9 pairs. Eachgroup was processed by using different methods to obtain cultures:
• (A) Explant-derived method, (B) Pronease-treated method.
, , epithelial cells. Most of the previous studies that were done to evaluate different corneal events employed animals and animal-derived tissues as an alternative model to human. Morphological, biochemical and species differences had placed such studies in a challenge. The availability of human corneal epithelial cell lines that retain their phenotypic trait and
• were species- as well as tissue-specific might make it possible to model
the human ocular surface in vitro.
I •
'i .'
'
. Cultures of h an corneal epithelial cells were obtained by using two methods of culturing, the explant and the pronease methods. The resulting cells were evaluated histologically and immunohistologically
.. regarding their viability, rate of growth, senility,....etc with the aid of
•phase contrast, transmission electron and indirect immunofluorescence
. .
mtcroscopies.
Materials and Methods:
Eighteen pairs of human donor corneas were obtained from the Michigan eye bank. They were non-reactive for hepatitis B, hepatitis C and AIDS viruses.
The corneas were divided into two groups each included 9 pairs. Eachgroup was processed by using different methods to obtain cultures:
• (A) Explant-derived method, (B) Pronease-treated method.
Other data
| Title | Ultrastructural and Immunohistological Characterization of Cultured Human Corneal Epithelium | Other Titles | الخواص المجهريةالدقيقة والهستومناعية لمزرعة النسيج المبطن للقرنية البشرية | Authors | Hala M. Fouad El-Mazar | Issue Date | 1998 |
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