EVALUATION OF SOME GENETICALLY MODIFIED BACTERIA ON ROOT-KNOT NEMATODE INFECTION IN TOMATO PLANT
GAZIEA MOHAMED SOLIMAN MOHAMED;
Abstract
Evaluation of some genetically modified bacteria on root-knot nematode in tomato plant.
Biological control of root – knot nematode, Meloidogyne incognita infecting tomato plants by genetic improvement of lytic bacteria, Serratia marcescens, Pseudomonas fluoresences and Bacillus licheniformis to increase the lytic enzyme, chitinase and protease production about several times than that producing by the wild types, were studied under both laboratory and greenhouse conditions.
The wild types of the lytic bacteria, Serratia marcescens, Pseudomonas fluoresences and Bacillus licheniformis and their ten mutants (overproducing chitinase and alkaline protease via using mutation), namely: mutant SM 190, mutant SM 167, mutant SM 36 and. mutant SM 21 from S. marcescens, mutant BL 3, mutant BL 132, mutant BL21, mutant BL 37 and, mutant BL 40 from B. licheniformis and mutant PF 63 from P. fluoresences which were produced by using mutagenic chemicals (MMS, EMS) and three fusants (overproducing chitinase and alkaline protease via recombinant strains on protoplast fusion technique) and the parents of two strains S. marcescens (mutant No. 18) and B. licheniformis (mutant No. 10), were evaluated against the root – knot nematode, Meloidogyne incognita, as safer and environmental friendly control alternative to chemical nematicides. They were evaluated as lytic bacteria (chitinase and alkaline protease) against juvenile survival of M. incognita under laboratory conditions by using standard (S) or (S /10), and exposure period (24, 48 and 72 hrs).
1- Laboratory tests:
Biological control of root – knot nematode, Meloidogyne incognita infecting tomato plants by genetic improvement of lytic bacteria, Serratia marcescens, Pseudomonas fluoresences and Bacillus licheniformis to increase the lytic enzyme, chitinase and protease production about several times than that producing by the wild types, were studied under both laboratory and greenhouse conditions.
The wild types of the lytic bacteria, Serratia marcescens, Pseudomonas fluoresences and Bacillus licheniformis and their ten mutants (overproducing chitinase and alkaline protease via using mutation), namely: mutant SM 190, mutant SM 167, mutant SM 36 and. mutant SM 21 from S. marcescens, mutant BL 3, mutant BL 132, mutant BL21, mutant BL 37 and, mutant BL 40 from B. licheniformis and mutant PF 63 from P. fluoresences which were produced by using mutagenic chemicals (MMS, EMS) and three fusants (overproducing chitinase and alkaline protease via recombinant strains on protoplast fusion technique) and the parents of two strains S. marcescens (mutant No. 18) and B. licheniformis (mutant No. 10), were evaluated against the root – knot nematode, Meloidogyne incognita, as safer and environmental friendly control alternative to chemical nematicides. They were evaluated as lytic bacteria (chitinase and alkaline protease) against juvenile survival of M. incognita under laboratory conditions by using standard (S) or (S /10), and exposure period (24, 48 and 72 hrs).
1- Laboratory tests:
Other data
| Title | EVALUATION OF SOME GENETICALLY MODIFIED BACTERIA ON ROOT-KNOT NEMATODE INFECTION IN TOMATO PLANT | Other Titles | تقييم بعض البكتريا المحورة وراثيا علي إصابة نيماتودا تعقد الجذور علي نبات الطماطم | Authors | GAZIEA MOHAMED SOLIMAN MOHAMED | Issue Date | 2014 |
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